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Add new ro-crate exploration tuto #5260

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3 changes: 3 additions & 0 deletions topics/fair/tutorials/earth_system_rocrate/faqs/index.md
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layout: faq-page
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154 changes: 154 additions & 0 deletions topics/fair/tutorials/earth_system_rocrate/slides.html
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---
layout: tutorial_slides
logo: "GTN"

title: "An earth-system RO-Crate"
video: true
questions:
- "How to generate a RO-Crate"
- "How to follow a Marine Omics study"
- "How to read a RO-Crate ?"
objectives:
- "Understand RO-Crate in a earth-system context"
time_estimation: 1H
tags:
- earth-system
- ro-crate
- ocean
- marine omics
key_points:
- "RO-Crate in earth-system analysis"
- "Marine omics workflow "
contributions:
authorship:
- Marie59
funding:
- fairease
- eurosciencegateway

---

# Workflow context


The workflow used as an example here goes from :
- First produce a protein fasta file from a nucleotide fasta file using Prodigal (it is a tool that predicts protein-coding genes from DNA sequences).
- Then, use InterProscan to create a tabular. Interproscan is a batch tool to query the InterPro database. It helps identify and predict the functions of proteins by comparing them to known databases.
- Finally, discover SanntiS both to build genbank and especially to detect and annotate biosynthetic gene clusters (BGCs).
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maybe you can also link the tools?

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## The workflow itself

![Marine Omics identifying biosynthetic gene clusters worflow image](./images/workflow_marino.png)
---

## Run the workflow

Import and run the following workflow https://earth-system.usegalaxy.eu/u/marie.josse/w/marine-omics-identifying-biosynthetic-gene-clusters
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You can now embedd workflows. Have yout tried if this is working in slides?

https://galaxyproject.org/news/2024-04-26-workflows-workflows-workflows/


To know more on how to do that follow the tutorial https://training.galaxyproject.org/training-material/topics/ecology/tutorials/marine_omics_bgc/tutorial.html
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Please try to use the link syntax.

Similar to The [Galaxy Core Architecture slides]({% link topics/dev/tutorials/architecture/slides.html %})

---


## Generate the RO-Crate

Once you run the workflow you can generate the RO-Crate. To do so follow the tutorial https://training.galaxyproject.org/training-material/topics/fair/tutorials/ro-crate-in-galaxy/tutorial.html
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### From workflow to RO-Crate

![From workflow to RO-Crate](./images/workflow_rocrate.png)
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### The RO-Crate folder and architecture

![From workflow to RO-Crate](./images/all_tree_structure.png)
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### Let's dive into the ro-crate-metadata.json

![Date of RO-Crate creation and other informations](./images/rocrate1.png)
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### Description of the RO-Crate structure

![Description of the RO-Crate structure](./images/rocrate2.png)
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### Galaxy Provenance

![Date of RO-Crate creation and other informations](./images/rocrate3.png)
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### Title and workflow details

![Title and details of the workflow](./images/rocrate4.png)
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### Dates

![Dates of running the workflow and creation of the RO-Crate](./images/rocrate5.png)
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### Datas

![Data uploaded for the workflow and produced by it](./images/rocrate6.png)
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### First tool Prodigal

![Details on Prodigal](./images/rocrate7.png)
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### Second tool Regex Find and Replace

![Details on Regex Find and Replace](./images/rocrate8.png)
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### Third tool InterProScan

![Details on InterProScan](./images/rocrate9.png)
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### Fourth tool SanntiS to build Genbank

![Details on SanntiS to build Genbank](./images/rocrate10.png)
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### Fourth tool SanntiS to annotate biosynthetic gene clusters

![Details on SanntiS to annotate biosynthetic gene clusters](./images/rocrate11.png)
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### Details on the different files in the RO-Crate

![Details on the different files in the RO-Crate](./images/rocrate12.png)
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### End of the ro-crate-metadata.json

![Details on the different files in the RO-Crate and end of the ro-crate-metadata.json](./images/rocrate13.png)
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### Thank you !

Try to do the same on your own workflow !
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## Shortcut Keys

- <kbd>?</kbd> activates help
- <kbd>Escape</kbd> leaves help
- <kbd>p</kbd> activates presenter mode
- <kbd>f</kbd> activates full screen mode
- <kbd>c</kbd> clones the slides into a popup

???

- The key to close the help isn't shown in the keyboard shortcuts, but it is the escape key.
- There are a number of other shortcuts which can be useful to know about.
- The clone window function creates a popup with a copy of the slides, this can be used to show the presenter notes on one screen, and the actual slides on another screen that learners can see.

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