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--- | ||
# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json | ||
name: "seqkit_concat" | ||
channels: | ||
- conda-forge | ||
- bioconda | ||
- defaults | ||
dependencies: | ||
- "bioconda::seqkit=2.7.0" |
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process SEQKIT_CONCAT { | ||
tag "$meta.id" | ||
label 'process_low' | ||
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conda "${moduleDir}/environment.yml" | ||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | ||
'https://depot.galaxyproject.org/singularity/seqkit:2.7.0--h9ee0642_0': | ||
'biocontainers/seqkit:2.7.0--h9ee0642_0' }" | ||
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input: | ||
tuple val(meta), path(input, stageAs: 'in/*') | ||
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output: | ||
tuple val(meta), path("*.{fasta,fastq,fa,fq,fas,fna,faa}"), emit: fastx | ||
path "versions.yml", emit: versions | ||
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when: | ||
task.ext.when == null || task.ext.when | ||
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script: | ||
def args = task.ext.args ?: "" | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
def file_type = input instanceof List ? input[0].getExtension() : input.getExtension() | ||
""" | ||
seqkit \\ | ||
concat \\ | ||
$args \\ | ||
in/* > ${prefix}.${file_type} | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
seqkit: \$(seqkit version | cut -d' ' -f2) | ||
END_VERSIONS | ||
""" | ||
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stub: | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
""" | ||
touch ${prefix}.fasta | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
seqkit: \$(seqkit version | cut -d' ' -f2) | ||
END_VERSIONS | ||
""" | ||
} |
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--- | ||
# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json | ||
name: "seqkit_concat" | ||
description: Concatenating multiple uncompressed sequence files together | ||
keywords: | ||
- concat | ||
- fasta | ||
- fastq | ||
- merge | ||
tools: | ||
- seqkit: | ||
description: | | ||
Cross-platform and ultrafast toolkit for FASTA/Q file manipulation, written by Wei Shen. | ||
homepage: https://github.com/shenwei356/seqkit | ||
documentation: https://bioinf.shenwei.me/seqkit/ | ||
tool_dev_url: https://github.com/shenwei356/seqkit | ||
doi: 10.1371/journal.pone.0163962 | ||
licence: ["MIT"] | ||
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input: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. `[ id:'sample1', single_end:false ]` | ||
- input: | ||
type: file | ||
description: Sequence file in fasta/q format | ||
pattern: "*.{fasta,fastq,fa,fq,fas,fna,faa}" | ||
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## TODO nf-core: Add a description of all of the variables used as output | ||
output: | ||
#Only when we have meta | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. `[ id:'sample1', single_end:false ]` | ||
- fastx: | ||
type: file | ||
description: A concatenated sequence file | ||
pattern: "*.{fasta,fastq,fa,fq,fas,fna,faa}" | ||
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- versions: | ||
type: file | ||
description: File containing software versions | ||
pattern: "versions.yml" | ||
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authors: | ||
- "@DLBPointon" | ||
maintainers: | ||
- "@DLBPointon" |
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nextflow_process { | ||
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name "Test Process SEQKIT_CONCAT" | ||
script "../main.nf" | ||
process "SEQKIT_CONCAT" | ||
config "./nextflow.config" | ||
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tag "modules" | ||
tag "modules_nfcore" | ||
tag "seqkit" | ||
tag "seqkit/concat" | ||
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test("sarscov2 and human primers - fasta") { | ||
when { | ||
process { | ||
""" | ||
input[0] = [ | ||
[ id:'test' ], // meta map | ||
[ | ||
file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), | ||
file(params.test_data['homo_sapiens']['pacbio']['primers'], checkIfExists: true ) | ||
] | ||
] | ||
""" | ||
} | ||
} | ||
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then { | ||
assertAll( | ||
{ assert process.success }, | ||
{ assert snapshot(process.out.versions).match()} | ||
) | ||
with(process.out.fastx) { | ||
// Including headers from both input files | ||
assert path(get(0).get(1)).readLines().any { it.contains('>NEB_Clontech_3p') } | ||
assert path(get(0).get(1)).readLines().any { it.contains('>MT192765.1 Severe acute respiratory syndrome coronavirus 2 isolate SARS-CoV-2/human/USA/PC00101P/2020, complete genome') } | ||
} | ||
} | ||
} | ||
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test("sarscov2 and human primers - fasta - stub") { | ||
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options '-stub' | ||
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when { | ||
process { | ||
""" | ||
input[0] = [ | ||
[ id:'test' ], // meta map | ||
[ | ||
file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), | ||
file(params.test_data['homo_sapiens']['pacbio']['primers'], checkIfExists: true ) | ||
] | ||
] | ||
""" | ||
} | ||
} | ||
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then { | ||
assertAll( | ||
{ assert process.success }, | ||
{ assert snapshot(process.out.versions).match()} | ||
) | ||
} | ||
} | ||
} |
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process { | ||
withName: SEQKIT_CONCAT { | ||
ext.args = "--full" | ||
} | ||
} |
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seqkit/concat: | ||
- "modules/nf-core/seqkit/concat/**" |
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--- | ||
# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json | ||
name: "tximeta_tximport" | ||
channels: | ||
- conda-forge | ||
- bioconda | ||
- defaults | ||
dependencies: | ||
- "bioconda::bioconductor-tximeta=1.20.1" |
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process TXIMETA_TXIMPORT { | ||
label "process_medium" | ||
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conda "${moduleDir}/environment.yml" | ||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | ||
'https://depot.galaxyproject.org/singularity/bioconductor-tximeta%3A1.20.1--r43hdfd78af_0' : | ||
'biocontainers/bioconductor-tximeta:1.20.1--r43hdfd78af_0' }" | ||
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input: | ||
tuple val(meta), path("quants/*") | ||
tuple val(meta2), path(tx2gene) | ||
tuple val(meta3), path(coldata) | ||
val quant_type | ||
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output: | ||
tuple val(meta), path("*gene_tpm.tsv") , emit: tpm_gene | ||
tuple val(meta), path("*gene_counts.tsv") , emit: counts_gene | ||
tuple val(meta), path("*gene_counts_length_scaled.tsv"), emit: counts_gene_length_scaled | ||
tuple val(meta), path("*gene_counts_scaled.tsv") , emit: counts_gene_scaled | ||
tuple val(meta), path("*gene_lengths.tsv") , emit: lengths_gene | ||
tuple val(meta), path("*transcript_tpm.tsv") , emit: tpm_transcript | ||
tuple val(meta), path("*transcript_counts.tsv") , emit: counts_transcript | ||
tuple val(meta), path("*transcript_lengths.tsv") , emit: lengths_transcript | ||
path "versions.yml" , emit: versions | ||
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when: | ||
task.ext.when == null || task.ext.when | ||
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script: | ||
template 'tximport.r' | ||
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stub: | ||
""" | ||
touch ${meta.id}.gene_tpm.tsv | ||
touch ${meta.id}.gene_counts.tsv | ||
touch ${meta.id}.gene_counts_length_scaled.tsv | ||
touch ${meta.id}.gene_counts_scaled.tsv | ||
touch ${meta.id}.gene_lengths.tsv | ||
touch ${meta.id}.transcript_tpm.tsv | ||
touch ${meta.id}.transcript_counts.tsv | ||
touch ${meta.id}.transcript_lengths.tsv | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
r: \$( R --version | sed '1!d; s/.*version //; s/ .*//' ) | ||
END_VERSIONS | ||
""" | ||
} |
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--- | ||
# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json | ||
name: "tximeta_tximport" | ||
description: | | ||
Import transcript-level abundances and estimated counts for gene-level | ||
analysis packages | ||
keywords: | ||
- gene | ||
- kallisto | ||
- pseudoalignment | ||
- salmon | ||
- transcript | ||
tools: | ||
- "tximeta": | ||
description: "Transcript Quantification Import with Automatic Metadata" | ||
homepage: "https://bioconductor.org/packages/release/bioc/html/tximeta.html" | ||
documentation: "https://bioconductor.org/packages/release/bioc/vignettes/tximeta/inst/doc/tximeta.html" | ||
tool_dev_url: "https://github.com/thelovelab/tximeta" | ||
doi: "10.1371/journal.pcbi.1007664" | ||
licence: ["GPL-2"] | ||
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input: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing information related to the experiment as a whole | ||
e.g. `[ id:'SRP123456' ]` | ||
- quants: | ||
type: directory | ||
description: Paths to subdirectories corresponding to | ||
sample-wise runs of Salmon or Kallisto | ||
- meta2: | ||
type: map | ||
description: | | ||
Groovy Map containing reference information related to the species | ||
reference e.g. `[ id:'yeast' ]` | ||
- tx2gene: | ||
type: file | ||
description: A transcript to gene mapping table such as those generated | ||
by custom/tx2gene | ||
pattern: "*.{csv,tsv}" | ||
- meta3: | ||
type: map | ||
description: | | ||
Groovy Map containing information related to the experiment as a whole | ||
e.g. `[ id:'SRP123456' ]` | ||
- coldata: | ||
type: file | ||
description: | | ||
Optional 'coldata' file equivalent to a sample sheet where the first | ||
column corresponds to the sample names (directory names in the input | ||
salmon/ kallisto results) | ||
pattern: "*.{csv,tsv}" | ||
- quant_type: | ||
type: string | ||
description: Quantification type, 'kallisto' or 'salmon' | ||
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output: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing information related to the experiment as a whole | ||
e.g. `[ id:'SRP123456' ]` | ||
- tpm_gene: | ||
type: file | ||
description: | | ||
Abundance (TPM) values derived from tximport output after | ||
summarizeToGene(), without a 'countsFromAbundance' specification | ||
pattern: "*gene_tpm.tsv" | ||
- counts_gene: | ||
type: file | ||
description: | | ||
Count values derived from tximport output after | ||
summarizeToGene(), without a 'countsFromAbundance' specification | ||
pattern: "*gene_counts.tsv" | ||
- counts_gene_length_scaled: | ||
type: file | ||
description: | | ||
Count values derived from tximport output after summarizeToGene(), with | ||
a 'countsFromAbundance' specification of 'lengthScaledTPM' | ||
pattern: "*gene_counts_length_scaled.tsv" | ||
- counts_gene_scaled: | ||
type: file | ||
description: | | ||
Count values derived from tximport output after summarizeToGene(), with | ||
a 'countsFromAbundance' specification of 'scaledTPM' | ||
pattern: "*gene_counts_scaled.tsv" | ||
- gene_lengths: | ||
type: file | ||
description: | | ||
Length values derived from tximport output after summarizeToGene(), | ||
without a 'countsFromAbundance' specification | ||
pattern: "*gene_lengths.tsv" | ||
- tpm_transcript: | ||
type: file | ||
description: | | ||
Abundance (TPM) values derived from tximport output without | ||
summarizeToGene(), without a 'countsFromAbundance' specification | ||
pattern: "*transcript_tpm.tsv" | ||
- counts_transcript: | ||
type: file | ||
description: | | ||
Count values derived from tximport output without | ||
summarizeToGene(), without a 'countsFromAbundance' specification | ||
pattern: "*transcript_counts.tsv" | ||
- transcript_lengths: | ||
type: file | ||
description: | | ||
Length values derived from tximport output without summarizeToGene(), | ||
without a 'countsFromAbundance' specification | ||
pattern: "*gene_lengths.tsv" | ||
- versions: | ||
type: file | ||
description: File containing software versions | ||
pattern: "versions.yml" | ||
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authors: | ||
- "@pinin4fjords" | ||
maintainers: | ||
- "@pinin4fjords" |
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