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A scientific publication describing the use of mScarlet fluorescence lifetime to quantify lysosomal pH.

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mScarlet fluorescence lifetime reports lysosomal pH quantitatively

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A scientific publication that describes a new approach for measuring lysosomal pH, based on the fluorescence lifetime of mScarlet. This repository hosts everything you need to reproduce our results. Read the publication here: https://jlazzaridean.github.io/mScarlet_lifetime_reports_pH (hosted via Julia Lazzari-Dean's personal GitHub account) or here: https://andrewgyork.github.io/mScarlet_lifetime_reports_pH (hosted via Andrew G. York's personal GitHub account)

This repository is intended to hold a (mostly) self-contained scientific publication. It contains:

  • HTML, javascript, CSS, and small images (index.html, appendix.html, and the javascript, stylesheets, figures and images directories)
  • A (limited) PDF version of the publication; note that this does not include animated/interactive figures.
  • Code which produces figures (in the figures directory). You can run and modify this code to verify and explore our results. See figure_generation/README.txt for instructions how to run the figure generation code.

Some files aren't suitable for version control, (e.g. raw data, minimally pre-processed data, etc). These are stored in a dedicated repository:

Hopefully this makes it easier to manage big, non-text files without risking the faithful version control of small, important text-based files stored here.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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