single cell multi-omics
Recent advances in molecular biology and genomics have enabled the development of powerful techniques that allow for the simultaneous analysis of a cell's epigenome and transcriptome. These methods have revolutionized our understanding of how gene expression is regulated and how epigenetic modifications contribute to cellular function and disease.
- Ma, S. et al. Chromatin potential identified by shared single-cell profiling of RNA and chromatin. Cell 183, 1103–1116.e20 (2020). SHARE-seq is a powerful method to profile gene expression and chromatin accessibility in the same cell; combining both layers of information shows that changes in enhancer activity can precede gene activation.
2.Zhu, C. et al. An ultra high-throughput method for single-cell joint analysis of open chromatin and transcriptome. Nat. Struct. Mol. Biol. 26, 1063–1070 (2019).
3.Zhu, C. et al. Joint profiling of histone modifications and transcriptome in single cells from mouse brain. Nat. Methods 18, 283–292 (2021).
4.Plongthongkum, N., Diep, D., Chen, S., Lake, B. B. & Zhang, K. Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2). Nat. Protoc. 16, 4992–5029 (2021).
5.Swanson, E. et al. Simultaneous trimodal single-cell measurement of transcripts, epitopes, and chromatin accessibility using TEA-seq. eLife 10, e63632 (2021).