diff --git a/lib/internal/test_runner/runner.js b/lib/internal/test_runner/runner.js
index 4c46e910800535..7443502848f930 100644
--- a/lib/internal/test_runner/runner.js
+++ b/lib/internal/test_runner/runner.js
@@ -596,6 +596,7 @@ function run(options = kEmptyObject) {
     teardown = undefined;
   }
   const runFiles = () => {
+    root.harness.bootstrapPromise = null;
     root.harness.allowTestsToRun = true;
     return SafePromiseAllSettledReturnVoid(testFiles, (path) => {
       const subtest = runTestFile(path, filesWatcher, opts);