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Hi, our fastq.gz data provider sequences one sample within multiple lanes and flowcells, and more than one sample can be present in one concrete flowcell+lane combination. For example, for 2 samples (D2077 and M245), pair end files look like this:
So the same lane number can be present in multiple flowcells (flowcells in my example are H2W7MDSXC,HFJHGDSX7,HFTFWDSX7,HWTLVDSX7). How should I correctly specify the lane column in samplesheet.csv? In sarek, lane can be a string, so in my case, I would for example for the first pair use lane = H2W7MDSXC_L2.
The text was updated successfully, but these errors were encountered:
This feature is now integrated into the raredisease project @sitems. Previously, lane information was not utilized for generating read groups, but I've now updated the code to include that functionality as well.
Description of feature
Hi, our fastq.gz data provider sequences one sample within multiple lanes and flowcells, and more than one sample can be present in one concrete flowcell+lane combination. For example, for 2 samples (D2077 and M245), pair end files look like this:
D2077_EKDN230048476-1A_H2W7MDSXC_L2_1.fq.gz D2077_EKDN230048476-1A_H2W7MDSXC_L2_2.fq.gz
D2077_EKDN230048476-1A_HFJHGDSX7_L2_1.fq.gz D2077_EKDN230048476-1A_HFJHGDSX7_L2_2.fq.gz
D2077_EKDN230048476-1A_HFTFWDSX7_L4_1.fq.gz D2077_EKDN230048476-1A_HFTFWDSX7_L4_2.fq.gz
D2077_EKDN230048476-1A_HWTLVDSX7_L4_1.fq.gz D2077_EKDN230048476-1A_HWTLVDSX7_L4_2.fq.gz
M245_EKDN230048452-1A_HFJHGDSX7_L2_1.fq.gz MM245_EKDN230048452-1A_HFJHGDSX7_L2_2.fq.gz
MM245_EKDN230048452-1A_HFTFWDSX7_L4_1.fq.gz MM245_EKDN230048452-1A_HFTFWDSX7_L4_2.fq.gz
MM245_EKDN230048452-1A_HWTLVDSX7_L4_1.fq.gz MM245_EKDN230048452-1A_HWTLVDSX7_L4_2.fq.gz
So the same lane number can be present in multiple flowcells (flowcells in my example are H2W7MDSXC,HFJHGDSX7,HFTFWDSX7,HWTLVDSX7). How should I correctly specify the lane column in samplesheet.csv? In sarek, lane can be a string, so in my case, I would for example for the first pair use lane = H2W7MDSXC_L2.
The text was updated successfully, but these errors were encountered: