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fastq-dump/fasterq-dump: can't open JTFH01xxxxxx as a RefSeq or as a WGS #946

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sa2adeel opened this issue Jul 13, 2024 · 27 comments
Open

fastq-dump/fasterq-dump: can't open JTFH01xxxxxx as a RefSeq or as a WGS #946

sa2adeel opened this issue Jul 13, 2024 · 27 comments

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@sa2adeel
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I am currently running sratoolkit 3.1.1 and have run into the following error:

fasterq-dump:

fasterq-dump --split-files path/SRR22287940 -e 6 --outdir test

2024-07-13T01:17:07 fasterq-dump.3.1.1 warn: directory not found while opening manager within virtual file system module - can't open JTFH01000001 as a RefSeq or as a $2024-07-13T01:17:07 fasterq-dump.3.1.1 err: sorter.c producer_thread_func: rec.read.len = 0
2024-07-13T01:17:08 fasterq-dump.3.1.1 err: sorter.c run_producer_pool().join_and_release_threads -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)
2024-07-13T01:17:08 fasterq-dump.3.1.1 err: sorter.c execute_lookup_production() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)
2024-07-13T01:17:08 fasterq-dump.3.1.1 err: fasterq-dump.c produce_lookup_files() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)

fastq-dump:

fastq-dump --gzip --skip-technical --split-files path/SRR22287940

2024-07-13T01:42:02 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000001 as a RefSeq or as a WGS
2024-07-13T01:45:03 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000013 as a RefSeq or as a WGS
2024-07-13T01:48:05 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000015 as a RefSeq or as a WGS
2024-07-13T01:51:06 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000017 as a RefSeq or as a WGS
2024-07-13T01:54:08 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000018 as a RefSeq or as a WGS
2024-07-13T01:57:10 fastq-dump.3.1.1 warn: timeout exhausted while creating file within network system module - can't open JTFH01000022 as a RefSeq or as a WGS
etc....

This error occurs in only a subset of the SRA accessions, specially WGS samples. The accessions were downloaded using prefetch and are unfortunately restricted as they are samples from a clinical study. As you can see from the files within the accession folder, the JTFH01 reference files are located within the folder so I am not sure why it is trying to download/create the files again. Also, our compute cluster is restricted from the internet so attempts to fetch any reference files via fastq-dump/fasterq-dump is denied.

JTFH01 KN707668.1 KN707750.1 KN707845.1 KN707912.1 NC_000005.10 NT_167218.1 NT_187387.1 NT_187443.1 NT_187508.1 NT_187545.1 NT_187582.1 NT_187619.1 NT_187656.1 NT_187693.1 NW_003315958.1 KN707607.1 KN707669.1 KN707753.1 KN707850.1 KN707913.1 NC_000006.12 NT_167219.1 NT_187388.1 NT_187444.1 NT_187509.1 NT_187546.1 NT_187583.1 ... NW_004166862.2 KN707661.1 KN707744.1 KN707835.1 KN707904.1 NC_000002.12 NT_167213.1 NT_187384.1 NT_187438.1 NT_187505.1 NT_187542.1 NT_187579.1 NT_187616.1 NT_187653.1 NT_187690.1 NW_003315955.1 NW_004504305.1 KN707662.1 KN707746.1 KN707840.1 KN707905.1 NC_000003.12 NT_167214.1 NT_187385.1 NT_187439.1 NT_187506.1 NT_187543.1 NT_187580.1 NT_187617.1 NT_187654.1 NT_187691.1 NW_003315956.1 SRR22287940.sra KN707664.1 KN707749.1 KN707844.1 KN707906.1 NC_000004.12 NT_167215.1 NT_187386.1 NT_187442.1 NT_187507.1 NT_187544.1 NT_187581.1 NT_187618.1 NT_187655.1 NT_187692.1 NW_003315957.1

I have been stuck on this problem for days now and can't find any resources to help with this issue. So, any help is much appreciated.

Thank you.
@klymenko
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What is the output of the following?
align-info path/SRR22287940

@sa2adeel
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Here is a subset of the output (let me know if you want the whole thing):

JTFH01000001,chrUn_JTFH01000001v1_decoy,false,remote
JTFH01000002,chrUn_JTFH01000002v1_decoy,false,remote
JTFH01000003,chrUn_JTFH01000003v1_decoy,false,remote
JTFH01000004,chrUn_JTFH01000004v1_decoy,false,remote
JTFH01000007,chrUn_JTFH01000007v1_decoy,false,remote
JTFH01000008,chrUn_JTFH01000008v1_decoy,false,remote
JTFH01000012,chrUn_JTFH01000012v1_decoy,false,remote
...
JTFH01001664,chrUn_JTFH01001664v1_decoy,false,remote
JTFH01001669,chrUn_JTFH01001669v1_decoy,false,remote
JTFH01001676,chrUn_JTFH01001676v1_decoy,false,remote
JTFH01001677,chrUn_JTFH01001677v1_decoy,false,remote
JTFH01001996,chrUn_JTFH01001996v1_decoy,false,remote
JTFH01001997,chrUn_JTFH01001997v1_decoy,false,remote
JTFH01001998,chrUn_JTFH01001998v1_decoy,false,remote
KN707607.1,chrUn_KN707607v1_decoy,false,remote:path/SRR22287940/KN707607.1
KN707608.1,chrUn_KN707608v1_decoy,false,remote:path/SRR22287940/KN707608.1
KN707609.1,chrUn_KN707609v1_decoy,false,remote:path/SRR22287940/KN707609.1
KN707610.1,chrUn_KN707610v1_decoy,false,remote:path/SRR22287940/KN707610.1
KN707611.1,chrUn_KN707611v1_decoy,false,remote:path/SRR22287940/KN707611.1
KN707612.1,chrUn_KN707612v1_decoy,false,remote:path/SRR22287940/KN707612.1
KN707613.1,chrUn_KN707613v1_decoy,false,remote:path/SRR22287940/KN707613.1
KN707614.1,chrUn_KN707614v1_decoy,false,remote:path/SRR22287940/KN707614.1
KN707615.1,chrUn_KN707615v1_decoy,false,remote:path/SRR22287940/KN707615.1
KN707616.1,chrUn_KN707616v1_decoy,false,remote:path/SRR22287940/KN707616.1
KN707623.1,chrUn_KN707623v1_decoy,false,remote:path/SRR22287940/KN707623.1
KN707626.1,chrUn_KN707626v1_decoy,false,remote:path/SRR22287940/KN707626.1
KN707628.1,chrUn_KN707628v1_decoy,false,remote:path/SRR22287940/KN707628.1
KN707630.1,chrUn_KN707630v1_decoy,false,remote:path/SRR22287940/KN707630.1
KN707631.1,chrUn_KN707631v1_decoy,false,remote:path/SRR22287940/KN707631.1

@klymenko
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What is the output of the following?
ls -l path/SRR22287940/JTFH01

@sa2adeel
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-rw-r----- 1 usr gdc 1322174 Jul 8 16:16 JTFH01

@klymenko
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What is the output of the following?
vdb-dump --info path/SRR22287940/JTFH01

@sa2adeel
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sa2adeel commented Jul 13, 2024 •
edited
Loading 

acc    : JTFH01
path   : path/SRR22287940/JTFH01
size   : 1,322,174
type   : Database
platf  : SRA_PLATFORM_UNDEFINED
SEQ    : 1,998
SCHEMA : NCBI:WGS:db:contig#1.1

Thank you for taking the time to help!

@klymenko
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Do all other files in SRR22287940 directory have the same permissions as JTFH01? ('Others' cannot read it)

@sa2adeel
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Yes, that is correct.

-rw-r----- 1 usr gdc    1322174 Jul  8 16:16 JTFH01
-rw-r----- 1 usr gdc      17184 Jul  8 16:10 KN707607.1
-rw-r----- 1 usr gdc      17188 Jul  8 16:10 KN707608.1
-rw-r----- 1 usr gdc      16836 Jul  8 16:10 KN707609.1
-rw-r----- 1 usr gdc      16774 Jul  8 16:10 KN707610.1
-rw-r----- 1 usr gdc      16701 Jul  8 16:10 KN707611.1
-rw-r----- 1 usr gdc      16685 Jul  8 16:10 KN707612.1
-rw-r----- 1 usr gdc      16830 Jul  8 16:10 KN707613.1
-rw-r----- 1 usr gdc      17206 Jul  8 16:10 KN707614.1
-rw-r----- 1 usr gdc      16909 Jul  8 16:10 KN707615.1
-rw-r----- 1 usr gdc      17203 Jul  8 16:10 KN707616.1
-rw-r----- 1 usr gdc      17356 Jul  8 16:10 KN707623.1
-rw-r----- 1 usr gdc      22556 Jul  8 16:10 KN707626.1
-rw-r----- 1 usr gdc      17150 Jul  8 16:10 KN707628.1
-rw-r----- 1 usr gdc      17004 Jul  8 16:10 KN707630.1
-rw-r----- 1 usr gdc      16912 Jul  8 16:10 KN707631.1
-rw-r----- 1 usr gdc      16744 Jul  8 16:10 KN707633.1
-rw-r----- 1 usr gdc      22488 Jul  8 16:10 KN707637.1
-rw-r----- 1 usr gdc      16749 Jul  8 16:10 KN707639.1
-rw-r----- 1 usr gdc      16837 Jul  8 16:10 KN707641.1
-rw-r----- 1 usr gdc      17161 Jul  8 16:10 KN707642.1
-rw-r----- 1 usr gdc      17140 Jul  8 16:10 KN707643.1
-rw-r----- 1 usr gdc      16683 Jul  8 16:10 KN707644.1
-rw-r----- 1 usr gdc      16921 Jul  8 16:10 KN707647.1
-rw-r----- 1 usr gdc      16801 Jul  8 16:10 KN707648.1
-rw-r----- 1 usr gdc      16869 Jul  8 16:10 KN707649.1
-rw-r----- 1 usr gdc      16795 Jul  8 16:10 KN707650.1
-rw-r----- 1 usr gdc      16898 Jul  8 16:10 KN707653.1
-rw-r----- 1 usr gdc      17122 Jul  8 16:10 KN707655.1

@klymenko
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What is the full output of the following?
vdb-dump -R1 path/SRR22287940/JTFH01

@sa2adeel
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ACCESSION: JTFH01000001
       ACC_CONTIG_LEN: 6
           ACC_PREFIX: JTFH01
          ACC_VERSION: 1
       AMBIGUITY_MASK: 0, 0, 0, 0
                ANNOT:
           BASE_COUNT: 4357270
       BIO_BASE_COUNT: 4357270
             CIRCULAR: false
             CLIP_LEN: 25139
           CLIP_START: 0
       CMP_BASE_COUNT: 4357270
         COLOR_MATRIX: 0, 1, 2, 3, 4, 1, 0, 3, 2, 4, 2, 3, 0, 1, 4, 3, 2, 1, 0, 4, 4, 4, 4, 4, 4
          CONTIG_NAME: decoy00001
CONTIG_NAME_ROW_RANGE:
               CSREAD: 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               CS_KEY: T
            CS_NATIVE: false
                DESCR: 49, 128, 182, 128, 48, 128, 162, 128, 48, 128, 160, 128, 26, 12, 72, 111, 109, 111, 32, 115, 97, 112, 105, 101, 110, 115, 0, 0, 161, 128, 26, 5, 104, 117, 109, 97, 110, 0, 0, 163, 128, 49, 128, 48, 128, 160, 128, 26, 5, 116, 97, 120, 111, 110, 0, 0, 161, 128, 160, 128, 2, 2, 37, 134, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 165, 128, 48, 128, 160, 128, 160, 128, 48, 128, 160, 128, 26, 4, 72, 111, 109, 111, 0, 0, 161, 128, 26, 7, 115, 97, 112, 105, 101, 110, 115, 0, 0, 0, 0, 0, 0, 0, 0, 162, 128, 48, 128, 48, 128, 160, 128, 2, 1, 17, 0, 0, 161, 128, 26, 31, 80, 111, 111, 108, 32, 111, 102, 32, 50, 53, 52, 32, 112, 117, 98, 108, 105, 99, 32, 83, 71, 68, 80, 32, 115, 97, 109, 112, 108, 101, 115, 0, 0, 0, 0, 0, 0, 0, 0, 163, 128, 26, 129, 154, 69, 117, 107, 97, 114, 121, 111, 116, 97, 59, 32, 77, 101, 116, 97, 122, 111, 97, 59, 32, 67, 104, 111, 114, 100, 97, 116, 97, 59, 32, 67, 114, 97, 110, 105, 97, 116, 97, 59, 32, 86, 101, 114, 116, 101, 98, 114, 97, 116, 97, 59, 32, 69, 117, 116, 101, 108, 101, 111, 115, 116, 111, 109, 105, 59, 32, 77, 97, 109, 109, 97, 108, 105, 97, 59, 32, 69, 117, 116, 104, 101, 114, 105, 97, 59, 32, 69, 117, 97, 114, 99, 104, 111, 110, 116, 111, 103, 108, 105, 114, 101, 115, 59, 32, 80, 114, 105, 109, 97, 116, 101, 115, 59, 32, 72, 97, 112, 108, 111, 114, 114, 104, 105, 110, 105, 59, 32, 67, 97, 116, 97, 114, 114, 104, 105, 110, 105, 59, 32, 72, 111, 109, 105, 110, 105, 100, 97, 101, 59, 32, 72, 111, 109, 111, 0, 0, 164, 128, 2, 1, 1, 0, 0, 165, 128, 2, 1, 2, 0, 0, 166, 128, 26, 3, 80, 82, 73, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 163, 128, 48, 128, 48, 128, 160, 128, 2, 1, 7, 0, 0, 161, 128, 26, 22, 112, 111, 111, 108, 101, 100, 32, 109, 97, 108, 101, 32, 97, 110, 100, 32, 102, 101, 109, 97, 108, 101, 0, 0, 0, 0, 48, 128, 160, 128, 2, 1, 10, 0, 0, 161, 128, 26, 19, 66, 108, 111, 111, 100, 32, 97, 110, 100, 32, 99, 101, 108, 108, 32, 108, 105, 110, 101, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 183, 128, 48, 128, 160, 128, 2, 1, 1, 0, 0, 161, 128, 2, 1, 20, 0, 0, 0, 0, 0, 0, 178, 128, 161, 128, 48, 128, 160, 128, 2, 2, 7, 222, 0, 0, 161, 128, 2, 1, 11, 0, 0, 162, 128, 2, 1, 21, 0, 0, 0, 0, 0, 0, 0, 0, 177, 128, 161, 128, 48, 128, 160, 128, 2, 2, 7, 222, 0, 0, 161, 128, 2, 1, 11, 0, 0, 162, 128, 2, 1, 21, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0
         EXTRA_SEQIDS:
         FEAT_ROW_END:
       FEAT_ROW_START:
       FIXED_SPOT_LEN: 0
              GAP_LEN:
          GAP_LINKAGE:
            GAP_PROPS:
            GAP_START:
             GB_STATE: 0
                   GI: 725026265
                 HASH: -604913549
                LABEL: contig
            LABEL_LEN: 6
          LABEL_START: 0
          MAX_SPOT_ID: 1998
          MIN_SPOT_ID: 1
                 NAME: gi|725026265|gb|JTFH01000001.1| Homo sapiens isolate Pool of 254 public SGDP samples decoy00001, whole genome shotgun sequence
       NUC_PROT_DESCR:
             PLATFORM: SRA_PLATFORM_UNDEFINED
           PROT_COUNT:
              QUALITY:
            RD_FILTER: SRA_READ_FILTER_PASS
                 READ: CCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGGGACAAACCACTGCGCCCGGCCAGAGCATATTTTAAAAGCTACAATAATTAAAACAATGTGGTATTGGAAGAAAAATAGACAAAAGTATAAAAAAAGTCAAGAAACAGCAGCAAATATACACAAGAATTTACTATCTGATAAAGATGACATTTTAAATAAGTGAAGAAAAGATCAATGTTTTTAAGCAGTGATGTCAGCCCAACTGGAAGAAAATGAGCTGGATTTCTAGTTTACACTGTATACCCAAACAAATTTTAGAGGAAACAATTATACATGTGAACAATGAAATAATCTTAGATGTCATACCTAAGAAATCTTTGCCTAGCCTTACAGAAAACACTAGAAGAAAAAAGGGTAGAATTTTTTTCATAATTGGAGTGAGGAAAAAATCATAAAACCTAGCATCCATAGAACAAAAGATTGCTACTTCATATATATATTTCTCATTTTTTGAACAGCTTTATTGAGGTGTATTTGCTATACAGTAACCCTTACATATATAAAGCAAATGATTTGATAGTTTTTACATATGTATACAGTTGTAAAACCACCATTGCAATCAAGATAACAAACACATCTGCCACTCTCCATGGCTCTTGCCCTTTGTAACCTGTCCCTCCAACCCCGCCCCACCCTGCTCCCCCATCCCAGGTGCACTCTGGTCTGCTTTCTCTCACTACTGACTAGTTTGCTTTCTCTAGAATGTTCTGTAAACAGAATCACACAGGGTATACTCTATCTTGTCGTATTTCTTTCACTCAGTAGAACTATTTGGAGATGTATCCATGTTGTTGCGAGTATCATTAGTATTTTCCTTTTTATTCCTGAGTAGGATTCTACTGTACAGATATACCTCCATTTGTTTATCCAGTTATCTGTTGATGGACATTTGGGTGCTTTGCAATTCGGGCTGCTGTGAACATTAAGCATACGTCACTGTATGGACGTGTGTCTTCATTCCTCTTGGTCTGTCTGAGCTCCACACCTAGGGAGGCTTTGGAATTGCTGGGCCATGTGATAAGTTCTATTCAACGTGTTAGAGAAATTGCTGAGGTGTTTCGCAAGTGGCTGCCCCATTTTCCGTCTCCACCACCAGCTTTTGATGGTTCTAATTTCTTCACGTCCTCACCAACACTGTTACTGTCTGTTGTATGTTTATTATTATCATTGTGGCCATGCCGGTGGGTGCAGTGGTATCTTGCTGTGGTTTTTATCTGCATCTCCCTAGTGACTAACGATGCCGAGCATCCTTTGAAGGCCACGTTACCATGATGACATCTTCTTTGGTGAACTGTCTACTGAAATCTCTTGCCCATTTTTTGTTGGCTCATTTGTCTTTTTATCATTCAGTTTAAGAGTTTTTAACATACTCTAGATGCAAGTCTTTTTTATCAGATATACGATTTGCAAATATTTTCTCCCAGGCTGTGGCTTGTCTTTATATTGTCTGAACAGTATCTTTTGAAGGCCAGAAGTTCTTAATTTTGATGACATTCAATTTATCATTTTTTCCTTTTGGTGTCATGTCTAAGAAATCTTTGCCTAACAACATAAAAACTTGAAATTTTTGAATTATGAAAAGGAATAGAGACAAAGAGAAAAGATAAATGAATAGATCAAAATATTTGAATTCATAGTATAAAGTGCTAATTTGTCTAATAGATAAACAGCTTTCACAAATCAATAAGCAAAAGGCCAGCCATCTGACAGAATGCATTGCCAAGGAATACAAGCAGACAGTTCACAGAAAAGGAAATACAAATGTCTCCTCCACAGAAAACTCAACTTTACTCATAAAAAGAGAAACTCAAAACTACAGCAAGATTTCATTGACTTAACCCTCAAGTGGGCAGAGATCAAAAAGTTGGGTGGCCACTGGGTTGGTAAGGAGGTAGGGGGTAGGAGATGGACTTTCTTACAGTGTCTGACAGAGCATGTATTAGAACAGAATCTACAGAGAGCCATTTGTCAATTACCTGTCAATACAACATTCCTTTTCATCCAAAATTGCACTTCAGGAAATTTATCTTTTGCATATACTGACTCATGTGCAAGATGTACGTGTTCAAAGATACCCATTGCAGCACTGGGTAAATGTGCAATATTGAAAGAAATCTATCAGCAGGAGACTGATCAGGTAGCGTATGGCTGATTCATACAATCAGACAATGGGACGTTGCTGGGGGAGTGTCCTCCCCTACCCAGGTTCAGGTTTCAATGTGCAGTAAGCCCCCTGCCTGGCTTTCTGTCCCAACCCCCAGTTAAAAAAGAGAAAGGGGCTCAGCTTCACAAATGGACGAATTAGAAGACACATTTCCAGGAGGTTCCCTCCTGTACCTTTTGAGTTTTATGCATATACTGTCATTCAAAACAAAGCAAAACTGGCTGGGTGCGGTGGTTCATGCCTAAAATCCCAGCAGTTTGGGAGGCCGAGGTGGGAGGATTGCCCAGGAGTTTGAGGTATCAGTGAGCTATGATTGTGCCACGGCATTCCAGCTTGGGTGACAGAGCAAGACCCTGTCTTAAAAAAAAAAAAAAAAGAGAGAGAGGGAAGAAATAAAATGGAAATCAGCATTTAGGCAGCGCTTTCAGACTGTGAGCACGCTGAAAGAAGAATGCACAATCATTAAAGAAAATGCACAATTATTCATCACCTACTTTGCGCTAGGCACTTTCTTCTAAGCCTCTCAACAACCCTGCAATAAGTGCTTATTATTCTTTACCATTGGGAGCCCAGGTTCAGAGCGGCAGAGCCCTTGCTTGAGGTGGCTCAGTTCTGTCTCCACTGACACCTGCCTAGGAAGCTTTACTTCCAACCTCCTGAACACACAAAAACACACTTTATTAGTACTTGTTTCCTTAATGCCTGCTGCAGGGTCTGGCCCTAATAATCATGCAGTAAGTTCTTGTAGGGTCACACAGAGGAGGTCAATGTTGGTGAGTCTCTCATCCCATCACATCGAGGCCGTTGTCCCCTGCTGCGCCCCAGCCAAAGGCTCTGCACATTACTCTGGGCAGAGATTGGAGATGCTGCCTGGGCCCTGATTTTGGACACCCAGCCCGGCACCATGTCGGGGCCATCCTACTCCAAAGTCCACCCCCTTCCTTCCCTGGGCAGAACCAGGAGCTCCATCTCTGTGGCCTGGGCTGAGGTGGGGGTCACCAGGCCCCTCACCCAGGCTTCCCTCTCAAGCACCAACATCCTAAGCCAAAGAAAACTATCCAAGGCTCTATTGTGGGGCTTCCTACAGCTCTTAGGGGAAGTGCAGATGAGGCACTGGGCCACATAAATCTTCTCCAGTGTCCTATAAAGCAGCTCACTTGGCCTCCACAGCTAGAACATTCCATCCCTGTCTCCCAGGCAGCCTCCCTTCCCCAGGATGCCCGGACACCCCTGCAGACACAGGCGGGCACACTCGCTCCCTCATGGCACTGTTACCACTGGTAGATAAGCTCTTCACGAGGAAAGGGACGCACCTGCCTTGTTACTACCGTGTGCAGGGTACCCGGCACCGTGTCTACAGTGAAGTGATGTAGGAGCCGGCTGAGGGATCACGAGTTTCCATTGCTGGAGGTTGAGGGCCCCCACCTGCCAGTCACACCAAGGCTGGTGAAGCTGCCAGCACCTGCCCAGCAAGGGCGGTGAGGCGCAGATCACTGACGGCCTATTTCGGAGCTGTTGGGTATTTGGTAGAACATTCCAAAAAGCAGAAGCTACCAGCAAATGTAGTCTAAGCCTTGAAACTTACTTTCGTTTTTGGATAAGATGGAGTCACCTTATCATGCAGGTTGCTACCTAAGAAAGACGCCCCTTCAGTAATCCCCACTTCTGTAAAACACACCTGAGGTTTCACCACAAAGCTCCCAAACACATTCGTTCCAAATTGCTTAACTACAGGATCTGCCAGAATCAGCCTGAAGCCCCGCAGTGGCGAAGGAAACGCCGACCGAGTCACCAGGAGGAGCACGTGCGGCCCTAGCAGGCGCGTCCGCTCCCGACTGCTGTGCACGCCGCGTGGGGCACGGGCTTTGGCGTCAGCGTTGCTCGTTTTGACGGGAATCGTTATCATGAAATCTTGTGACACCCACGCCTTGTGCCCTGGTTCATGCAGAGATTAAGAAAAAGCTCCTTACTGTTCATTATTTAAATTAGCCGGTCGGCAACGGCTGGAAGACACCGTGCCCGCCAGTGAAGAACAATGCCAGGCCGGCCGCGCTCACGCCTTCCCACTAGAGGCGGGCCTGGGGGCAGGGCGGGCCTGGGGGGCTGCTCACAGCCCTGGGGGCCCCTCTCGGTGGAGGACCCATTTCCTGTTGTGGAGATTCTTCCCTTTGCAGCCAGGAGAGGGGTCCGGGAGGGCAGATGTGTGCACAGCTCCAACTTCTTGTTTCTCCTTTTCCACGGAGAACATCGACGGAGCGGAAATGTTTGCCGCACGCAGAAGCCTGCAGCTGCATCTCCTCTCCGCCCGCTCCTCGCCCATCAAGCCTTATCGATCTGCAACTGCATTTCGCGCTGAGGTGAAAGTGTTGCGCGCTGATGTGGAATTCTAATCTTCACCGCCCGCTCCCCGCCTGGCCACGGGCGCCCCTCGCACCCCTTCCTCCCCACCCAGCCGGTCCGGCGTGCCTCATTGCAAGTAATTATGCTGCACATGAGTCATTTTTTAATGTTTGGTTTGATACATACAGATTCTTTAAACAATAAAAAAAAATCAGCAAACAGCATCACAATCTGGAAATCTCTTTCTTAACATGGAAAACGGTTTCTTAATGGCACAGGGGGGAACTCAACAGCAAGCTTGACAACATAATGAGGAGATTGTTCCTGGGAAGGGAAGCGCGTTCTTCATGCAGCTCTTACTACTCTCAATAAGATTATTCTCCCTGAATAGCTAATCAGCTTTCTCCAGCTCCCCAAATCCGCGTGCTGGAGGCTGCTCACACGGCTCTCCTTACGCACTAAGTGGCACACAGTGACACCTCGGAGGGCGAAGTCTAAAGTGTTAACAGTATGTGAGCAAACAGCGCAATGGAGGTTGCAGAGAGAGAGGCCTTTGGCAGATGAGCAGCAATTTAGGAAGACGGCTCTGTCACACCTGGGTTGACAACGCTGACAGGGTTTGTTGATCTCTGCTCCCATCTCGAATTTGAGTGTCACGTTTCAACAGAAACGAGAGAAATTAAAAAAAAAAAAAAAGCTTTCAAGAGTCAGCGTTGTTTGCACGGTGTTTGTGACACCGAAACACAAGAGCACGGGGTGGGAGCTGCACTGACCCCGCCTGCAGCTACCCCCACCCTGCCAGAGCCCGCAGAACTGGGTGCAGGGTGAGGCCCGTGGGTCCGGAACAGGGCAAGTGCCCACGGCTGCTCGGGCCCCTCCGGAGCATGTGGTCCTGGACAGTGACACACTTCAAGCCCTGAGCTCACTTGGGAATAAAAGGGCTGATCACAGCCTTGCCTCTGGTCCCGTCGTGGCCCCCACGTTGTTGTCTCAGGGCTTCCGTAGAGGTTGCTCTTCAGGACAGATGACAGGGACCCCAGAGGAAGGCCTGGCCAAGCGTGGGTGTCACCAGACTGCAGGGAGTGAGAAGAGCTGCTGGAGACTGCCCGGCAAGCTTGCCTGGTGCCACAGCCCAGCACATGCCCCACTTTCAAGCAGGTGGCGTCAGACTGTCTGTAGGGCGTAAGAGCGTCCTGGCTCTGCAGGGGTGGCTGAAGCCATTGCGGGCATGTGGAGCCTCATGGAGACGGTAGCCATTTGGGTCCTGAAGCAGCTTCCAGCCTGTCTGGAGCTCTCAGAGTGCCACAAGCACAGATGCAGGGCGTTCTTGACAGCTCACTGAAGACGTGCGTGTGCATCACCCCACGGGTCCCTGGGGCTCCGGGCGTCCCATCTCCATGTCCAGGGCCAGAGGGAGAGGAGGCAGAGCCAAGCCCAGGTGTACTCTAGTGACCCACAGCCCTGAAACACCATAGCCTCATCCAAAGAAAAGCAAGGACACTTGGTGCCTGCAGAACTGAACTGCGCTGGATCCCGCCTCAGAGGCTGGAGAAGAAGGCACACCGGTGGGGCCAGCATGGGGGCCAGATAGCCCTGTCCTTGCCCCTCACTCCGCCTGACTGAGCGCCTCTCAGTCCAGGCAGCAGGAAGCCTCTTCTCCTTGGGAAGCCACTGGCAGTCAGGCTGGCTGTGTGGGGACTTGGCTTCATGCTCCAATATTTGGAAGCTTGATAGAGAGCTTTCACCCTCTACTTGCTTTCTTCCACGTGAGCCCCGTTTTCCCCGGGTGAGAATGCGCTTTGCCGTATTCAGGGTCTGAGCAGCATGTGCCCATATCCTCTCTCCCAACATCCCCATGTCGGGCCCAGAGAGTGCCAGGGCCTGTGGGCTGGGAAGCGGTCACGCCCAGGCGCATTCTGCCCATGCGGCTAGTGCCGTCTGAGAGAATGCACAAGGCGGCCTGCGCTGTGGGCCCTCTGGTCCCAGAGCCACAGTGTGAGTCATGTGCTGCCTCATTGTGAAATGCCCCTGCCCTCTGCCCGATGCAAGGCCCTGGGTGCACGCATGACTCATGGTGGAGGCAGGAGGAAATATGCAAGTTTCTCCACGAACCGGGTGCAGGTGGTCGTGACCTTAATGCCGTTTCCACAGGGGGCCACAGAGACATTCTTTGCAGATTTTTGTGAACACCGTACTCTTGGAGGCCTCCACTCTCAAGCTGGTCCCCAGATGGGCCCGGTAGAAACTCACAGCAAACATGTCTTCCCCAAAGGAAGAATGACAACAGCTGTCTTATTTTGGTGAGGGTAGTTTACAGGGTAGTTTTGGTTATGTTGCAGTGACCCCAAGGTCAGAACCTCAGAGCCGAGATAACCGTTGGAGCTGTGCTCCCCACGCTGGCTCAGCCCCTGGAAGCCTTGGTCTCATGGCTGCCCCGTGTCAGCCGATGGTTCCCAGATCATCAAGACGGTAAGCAAAGCGATGTGGGCACTCCAACCCGCCAGGTCCTCAACATGTCCATGCAGGAGGTACACATGTCCACATCCCACAGTCACCAGCCCAGCCTGGCTTAGCTGAGAAATGCAGCTCTTGTCTGCATGCAGGAGACAAGGCCCAGCAATGAGTCTGAGCAGCACCAAGGTCCGTCCTGTGCGTCAGCTCCCAGGTCTCGGCTCACTGTGCAGGAACCGGAGCTTGATATTTACGACATCTCCTAGGTCATGCACTGTTCATTCATCCGAGGACTAGTTATCGGGAGCTTGTGTTCCTGGAGCTGTGCTCTCCCCTTGCCAGCCTCAGCTCCTCTTCCACCCCAACCTGTGCCCACCACCCCCACACCAGAGGGAAGAAACAACCCCGACCTCAGGTATAGAGTGAAGTCCGGCTGATGCTGGGCTGAAAGAGTAGCTGGATTCTTATTAGGACACAGTAAATGGACCGCACTCAGGAACACGCTCACATCCCAGTCTCTGGATATGGAGGACTTGTGTGTACTGATTCCCCCAGAAACTCATGGACCATGAAAATGACTAAGTATCTCATCTGGCAGGATTTTCATTTGCTCATTGGTATTAGGACAAACAAATGGAAACATCTCCTCTTATTATTCACAGCAAATATGTGGCCACTGCTTTCCTCCCTAACTTCTCAGGCTTAACTGTAAATGCGAATCTCCTGGTGTTTCCTGGAGCTTTCCCCTTCCTTTGTACCATAGGCCCCACATGTCACATTCCTCTGGCACCTCTTTTCCTGCCTTACAGTTACTATCGGCCCTTGTCATCCCCACATCAGGACGCTGAGCTGCGCATGCTGTGTCTGGGCCACGGTCCCCATGAAAATCCTTCCCCTGGTGTTCTGGGTGCCGCCCTCCGGCTGTGCCCTTCCATCCTGGGAAGGTCTGACTGGGGTCCCACGTCACCACGTGGTGGGCCCCACATGCCAGTTCACTCTCGGCTTCCCAGCGCCTCTCTTCCTGCTGCCCTCACCTGCTCTTCTGAGGCTCCTGCAAGGCCCAAGATCCGTCTTTGATGACATTTTTCCTGCTCTCGTTCCTGGCTACAGACTGTTGCCATTTTCCAGCAATGGTTTGAAATAGCATTAGAAAGTTTCATCGAGAACAGAATTCTCTGCCCTACTTCAACATTACGTGTAATCCTATTCTCTGTGAAGCCTCCCCAGCATCACCAGTGGCCATGGAAGTCCCCATCCATGAAACTCCCATGTGGAAACTCCGTAGAGAGACCTCCCATCCCGCTGTGAAGTTCCTGCGTGTCAATGAGAGGCCGGGTTGGGGTAAGATGATCTGCATTGATTTTGAGTCACTGGATGGGAAGACACCAGGGCTGAGATCATTAAGGAAGCTTATTGCTCCGGAAATACACCTGGGCAGAGGCATGTTGTTGACCTCAGAAGCCACGCTGCCTTCCCACACCCTGGTTATTTAATTTTCTTTTGTACTAACTAAAATCAGATTTCTCATTGAGGGTAGAATTTACTGACTTTTCGGATAATACGGAAAGCACACGGGAGGGCTCACCTCATCCTCTGCAATGAGGTGTGCGCAGCATCAGGGTCTGTCTAACCAGGGTCCCCACGGCCAAGCCAAATGAGAGGAGTGCAGACTTCAACTCACAGCAAACCAGCTGAACCCAAGCCTCAAGAGCTTAAAGATTTTGTTAACCAAGATCCACAGACTGAGGACAAGGCAGTTATGCGCTTGAGCTGGCAATAGCCACTGAGCTCAGCGCTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGATGCACAGGGTGCGGGGCAGGAGCGTTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGATGCACAGGGTGCCGGGCAGGAGCGCTCAGCCTGGATCATAGACGGCAGCACCACCATGCTCGGATGCACAGGGTGCCGGGCAGGAGCGTTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGATGCACGGGGTGCCGGGCAGGAGCGCTCAGCCTGGATCATAGACGGCAGCACCACCATGCTTGGATGCACAGGGTGCCAGGCAGGAGCGTTCAGCCTGGATCACAGATGGCAGCACCACCATGCTTGGATGCCACAGGGTGCCGGGCAGGAGCGTTCAGCCTGGATCACAGACGGCAGCACCACCATGCTCGGACGTACGGGGTGCCAGGCAGGTTTGATGTTTCTGTTGATTTTGGCTTCAGCAGTTGAACTCTGGCCTCCATAGGAGGCCCTCCGAGGCCGGAGGGGCCCACACCCTGTATTCCTGCACTTAGTGTCATCCCCGGGCTGGTTAGAGGCTGTCGAAGGTTGGTGAAGCCCTTTGAGCCATCAGGCTGAATCAAGCCTGCAGTGACTTTGTCATCTGTGAAGGATCCTTTCACGGTGAACAAAACTTCAGCTCATCCCCAAACAGACAGACAACATGCTCCTGAGCAGATACGGCATCCTCTGTGGCTCTCCACGCACATCGGAAACATTTTCCTTCAAGCGAGATGAAAATGTGGCAGCTACGCCAACAGCCAGTGCTGAATTCTGTCTCCAGCGAAACGAGGATGGGTGGCTGAAATGGACAGATAATCTAAAAAGGCAAACTTTAGAACACAGAGTATCAATGTGTTCCAAAGCAAACACTTCTTAAGTACCTGGATTACAGGACTTGAATTTCATTTTCTTGTCTTTAGTGCAGAGGAAGCAGACAAGAGAAATGTCTGTGTTAACCTCAAAGATGAGAATTTGGGGTCAGTTCTCAGGGGACAGCCTGTGGCCTGTGTAAACCTCGGCAGTTTCCCTAATTTCTCCCAGCCTCTGTTACCCACACGGCCACCCTGGAAGGAAGACGGGAGGGGTTGTTGGATAGAGAGTTCGCAGTGCGTTAATGAAGCACTCTTGGAGCACACGGGAAGGAGGGATTAGCTCTCACATTGAAGGTGGCATGAAGGCAGGTGCCAGCATGAGGAGGAACGGGAACCCCAGGTAATCCTCCAGTGGCGTCACCTGCCCTCTGGATAACTGAGAGGTGACTAATCTTCTGTAAGTTAATTGTCAGGTGTGGAAAGATTTTGCCCACGGTGAGAGTAGCTCCCGTACGGCACAAAAGGACGGCTTGATTTCCTGCACTTAACACGAGTCACTAGGAGAATCATCTCATCCACCAGCTTCTCCAGTTTTATCAATTATTTATTATTTCCCCCATGCCAAGTTACTTCCATGCATTCCACCCTGTGACAATCACGGAACGCCTGGCTCCTTGTGGCCTGGCAGCACATGGGGCGGCTAGCCCGGACCCTGTGCACACCACCTGGCACGGGACCTGGGGTGGGTGTCTCCCCAGAACCCAGCCCTGACCGGTGGCAGCATGAGGGCCGGTGACCTCTGCGAGAAGCTCGGGGGGGCCTTGCGGTCAGGTCATGTGAGCATGGTTAGCCAAGTGGAGAAGAGCAAACTTCTGGATTCCTAATCAATTCACCTGTTTTGAAATAGAGAGGGATGGTCCCACGGTGAGCACACGGGGTGGGCGTGGCCCTGCTCAGTGCAGGTGGCCAGCCTTGGACTCACACTGGGTGGCCCTCCTGTTCTCCGGGTTCCGCTTCCCAGGCTGTTTCTGTCATTCTGCACCAAAGACCTTCATTCCAGGAGTTACCTACTTAGGCTAAATTGTGAGAATAAAGTGGAGATGGTAGAATTTCCCAGGAGCCGTGCGTATCTTGGGGAAGCGTTTCTTCTCCCAGCCTGTGTCCAGCGTGGGTGGGCTGCGTGGCTTTGTGGATGGAAAAAAGACCCTTCCCATGAAACGGGAGCCCCTCCCCTTCCAGGTTGCTGCAGTCTGTCCTCAGAGAGCCATGCCTTTGACTCACTTTAAATGAGATTTTCTCTTCCCCTGAGGAGAATCATTAACAGATTAATCTAAAAGATTTTCAGAATCTTGCTTGAAAGGCAGATTTTCTTAGAAGAAGAAAAGGAAAAAATAGAAAATTCTGGGTTCCCAAGGTTGGGGGTTGGGAGGAGGGGGCATGGCATCCCCAAAAAGTCAAAGAGTAGAAAAAGAGATGCCGGTGCACCTCCAGGGAAACGCACCTTAACTTGGATGCTCCTTGGTATGTATCCATGACGAGAACAGTGAAGGGCTTGACAATGGGCCAGTCCTACATGCCTGCAGGCCACACCCCCATCCACTGTCAGCAGATCCCCTCTGAGGTCTCCTGACGCCACTCAGAGCAGGCCAGACTCTGTCAGTTCTTCTCTCAGTTCCTGGCATCCTGCACCTGAGGCTTTGAGCACAGCTCAAGTCCCCTCTCCCATTCAGGTTTCCTGCTTGCTCCAACCAGACCCGTTTGCCGAATCCCCTGGGCCTCGCATCCGTGTTTCTATCTTGGCAGTCACCTTCTCTGTCTGGCTGCCCCTCCCCTCTTCCCCTGTGCAGTGGGAACTCTAGATGCCTGTTGAATTGCATTATCATATTCAATGATGAAATTGATGGACAGCAACAACACTCAATGATCAGCAGTAAAATGAAGAGTTACTAACTTTATGGTTAGGTACTTGGCCAATACAATTAAATAATAATTTCCTCCTTTGTATGTGCACCACAATTAAAGGATAAACTCTGCATCAGCAAATGGAGTGTTTTCAGCCAAAGTCAGTCTTAGTCCTGTGACACCGTGGAGCTGGGGCAGACCACCCCCACCTTCCTGGGAGGCTGCACAGGGAAATAGAGAAAACACGGAGATTGGCATAGAGATTCCTTCTCATCCTCAGGTTCCTGACATGCAGGCTGGATAGGACACCACCTTTCCTGGCCATCCTGGAGGGCGGAAAGCGGTAACTGGAGTAGAGTGCTCAGTGACTCATGGGCATAGGTACCCATCACACGTGCAGTGGAGATTTCATTCCCCGGGCTGTCCTATCAAAGCACAGACTGGGTAGCTTAGTACAGAAATTTATTGTCTTGCAGTTCTGGAGGCTGGAAGTCCAAAATTAAGGTGTCGACAGGGCACTGCCCTCTCTGAACCCCCTGAGGAGAATTTTGTCTTGCCTCTTCCAGCTTCCCGTGTGGCCGGCGAGCCTTGGTGTTGCTCAGCTGGTAGACGGTGTCTTCTCCCTGTGTCTTCGCATTGTCTTCTTTGCGTTCCTGTGTCCAGACTTCCCTCTTTCTAAGACACCAGTCACATGGAATTAGTGCTTGCCATAGTGACCACCTCTCAACTTGATGATCTCTGTAAAGTCTCTATTTCAAATAAGGCCACAGGCACAGGTTCTGTGGGTTAGGACTTCAACATAACTTTTTGGGAGGGGATGCAGTTCAACAAATAACAATTGGCTCCCTGACCAACCTTAATGTATTCCTCTCCCCACCTACCCCAAACACTGTGCACTGTGAGGCCAGGTGAGGTCAGTGGTTCTCAAACTTTTTTGTCTGAAGAACCTTTTACACTATTAACAATCACTGAGAAACAAAATGCTTGCTTTTCCCCTGCAGGATATATCTATTGATATTTACCAGGGTAGAAATTAAAAATAGGAAAAATTTTAAATATTCATTTTTAATTAATTAAAAAAATAATAAACCCATTACAGATGCCCCTTGACTTTTCATGGGGTTATGTCCTAATAAAACTATTGTGAATTGAAATTATAAGCTGAAAATGCATTTAATACACCTAGACTACTGAACATCACGGCTTAGCTCAGCCTACCTTAAAGGTGCTCAGAACACCCATGTTAGCCTCCAGTTGGGCAAAATCATCTCACACAAAGTCTATTTTATAATAAAGTGTTGAATAGTTCAAATAATTTGTCGAATACTGAGCTGCAAGTAAAAAAGCAGAATGGTCCTATGGGTGCACCTGCTATGGTCTCTACTGAGCGCGTGTCACTTGAAACCATCCTAAAGTTGAAAAATCTTAAGTGGAACTACTGTGAAGTTGGTGAACATCTGTGTGGAAGTAACTGTATTGTTTGAAAAGGAGTGAGAAGAGCAGCATGGCATCACATTTTTAGAAATCTCTAATATCTGGCTTAATAGAAGTTGGCTGAATTATGATAATGGCTTCTTTCAATCTCTTGCAAAACATTGTTTTGGGGGAAGTATAGGAAGAAAACCAGCTTCTGGCAGATGTGTGTGGAAAGGGAATGCGTATTTTTATAGGCTTTTCCAATCATTATGGATATTCCTTGTGGCTACAAAAAAACTTGAAAAATGGTAGTGCTATAAGATTAGTTGTGGAATCTAAAGCCACACCAGTTCCCTTTTTCACTCTGTTACATCACAAGTCATCAGTCTGTTTGCATGCATGATTTTGTACTTGGACACTATCATTAAAAAATATTGTTTCAGTAAATTTTATACACACACACACTCACACATATATGTAGAGACAGAGAGTGCGTGCACGAGAGAGAGATGGGATTTTTCTCTGTCGCTGAGGCTAGGGTACACTGGTACAATTGTGTGATCATAGCTCACTGCAGCCTTGAACTCCTGGGCTCAAGTGATCCTACTGCCTCTGGCTCCCAAAGCACTGGGATTACGGGTGTAAGCCACCGCATCCAGCCTAGACAATATTTTGAAAATTACATTTGTTAATACCACACATCTCATCAGAAAAGTCTTTAGATCGAGAACATTTCAAGCTCATCGTGGCGGGTATGTTTTCCAAAACTCTAATTTTCACTTGAAGGCTGAAATTTATTATTGGCAACAAATACTGTCAGTTATTTTCTTTGAAGTGATGTTGTGGAGAAAATAAGTAGTGTGCGTTCGTTAAGCATTCTTTCAGACAAAGATGGGATTTCACGGAGAGTGGACAGTGCAGGATCCAGCTGGAACAATCACACGTGCCTCCATCCCTCAGTTTGCAACAGCTTGCACTCCCCATCTTCACATGGGATGTGGGAGAGACAAATACACAAGGGCCAAGGTCTAATACGTTAAAAGCTTTCAGTGCTTCATCCAGAACACCCTATGTGAAAGTGGCTTTTTTCCTGCAAGCGCGTGGTAGGGACCCATGTCGTGGCTCCTGGTGTGGTTGGGCGCAACCACGTTGGCGGATGCTGAGCTGCCATCAGTGTGAACGTCAGTTCGATGAGAGGGTGCCTGTTGAAGTAACATTGCTGCACAGCAAATTACCACAAACGTAGCGGCTACAAACAACCCACAGGTGCCATCTCAGAGCTTCTGTGTGGGTGGCCGAGGCTTAGCCACACCCTCGGCAGGTCTCCCCCGGCTGCAATCGAAATGTCATTTGGGGCTGTAATTCTCATCAGGTCTCAGGTGCGCTTCCAAGATCACATGGACAGTTGGCAGACCTCCGCTTATTGGGGTGATGGCTGAGGACCCTGTCCTCTTGAGAGCTGTGGGCTGGGGGCCGCTCCCAGCTCTGAGATGCCCCTCCCTGTAGATGGCTCACACCATGGCTATTTACCTTTTCCTTTCAAGAGAATCCAAAGAAAAGCTCTGCTTTCAAAGGGCTCACCCGATGAGGCTAGGCCCATACCAAATCATCTCTCTCGATTAATTTAAAGCCAACTGATCTGGGGCCTTGATTACATTTGCAATGTTTTGCCACTTAACATGATCACAGGAGTGATAGCTCATCATTCCAACCCTGACTCAAAGGGAAGGGACTCTACAGGGAGCCTACAGCAGGGTGAGGGTGGAGGAATCACAGGGCTCTCCTAGAACCCTCCCAAGTTTTAAGTCAATACTACCATCGGGACTGGCTACGTAATTTCCAGGGCTCAGTGCAAAATGAAAATGTGGGGGCTCTTCTTCAAAAATTATTAAGAATTTGAAGACTAACAGCTGAGCATTAAAACAAGCATAGCACCTTCTATGCCAGGATCCTGGATGTCTGCCCAGGTCACAGGCTCATGAACATGGCCCTGACTTATTCGCCAACAGTTTTGGTCTTGCAGACACCTCAAAAGGTCCCAAAGACCCTGGGGGATTTGTGGGCCATCTTGGAGAACTGCTGAGTCAAAGCAGCTCAATAATTGTCTGTGGGGACATTTGTCTGACCTTAGTCATGTAGGAAGTCTAAGAAGTTTCTAGCAGCAGTGAGACATCACAGGCCCTTCTCTTTTCAGAAGTCCCTTCTTATTTTCCCTATAGCACAGGACAATTTCCCCCAAGACCGGGCTTGTGCCAGTGACTCGATCACACAGGTGCCCAAGTTTAGATCTTTTGTGATGGATCCCAGGTCTCAGAAGCCGGGGTCTGTGTGAGCATCCCCATCCTCAGGACGGCTCCCAACGTTGGCCCTGCAGCTTCCAGAGATGGGGGTGACAGCAGCTGCTGTGCTGGTGGGAATTTTAATTTTTTTAAATTTTAAGTTCTCTTTTGATTTTAATTTTTTAAGAAGTTAAAGTTGTGTATATTTACTGTGTATGACATGATGTTTTGAAACACATATACATGGCAAAATGATGATGGCATTCAGGCTAATTACCCATCCATGACTCCTGGAGGGGATTTTAATGTGAAACTGTCCCAGAAAACCTCCCTGGCTTCTGGAGAAGATGCCTTCTTAGAGAAAGCACATCCTAAGAAAGTCTTCCAGAACTAGAAGTGGACTCTTCTGCCAGCACCTCAACAAGGCTTCCTATCCTTCCCTTTATGCTGGGAGCAGGTGGCAGAGCCTTGGGGACACTTCGTGCCTCCTCCCAGGAAGAGGCTCTGGGCACATTCCTCATCCAGCAGTTTTTCCTGGTGATGCATGGGCCCCTCTGCTGACTCCGGTCTTGGAAGCCGGAGACTGTGTGAGTGCGGGCTCCGGTAACACTCATGGTCCTCAGCTTCTGCTTTCCAGGGCTTGCTTCACCAGGAGGGACTCCATGTTGAATGCGTGGCCTCTCCTTGTGAAAAGCTGCACTTTCGGAAGCTGGTCCACACCAGCCAATGGGGCCGGGACCTCCTGCTGTGATTCTGGGCCTTCTTTCCCAGAGTTCACCTAATTTATCACAAGTTATTTTTGCCTGGAAGCCTGAGAAATCCTTTTACAGTGATAATTTCACTATGGCCACCTCTATCTCTGCAGCCTCCAGGCCTGCCTGGTCATCAAAGCCCACCTCATTGCAGATGGTCACCTAAGCCCACCTCATTGCAGATGGTCAGCTAAGCCCACCTCATTGCAGACCCAAAGCCTCCACCTCATTGCAGACCCAAAGCCTCCACCTCATTGCAGATGGTGAACTTTGTCATGATGCAAATTTTGCCACGTTCCTCTCCTGCCTAAAATCCTACAGTGGTTCTCCACTGCATTAGGTCAACAAATATTTACTGGAGACCATGACTGTTCTAGCCAGCAGGGATACATTGGTGGACAAAGCAGAGAAGCATCCCTGCCTTGTAGAGACGACATTTTCAGGGAAGTCAGACACCAAACAAGGCATAAGAAAACCGCAGGGCGTGTTGGAAGACTCATGTAGTGGGAGGGTGGACCTGGACACGGGAACCCGGAGGCCTAGGGGATGGGCACCGCAGAGTGGAGACCCCCCTGAGAGGTGGCGTTTGGACAAGGACAGAGAGTGAGGGAATGGGCCCATGTGTGTCTGGGGAGATGGCAGGTGAAAACCAAGGTGGCACCCACGTGGCTCCTGGAGGATGTTGAGCCAGGTCAGGGCAGTGAGAGGAGATGTGGTTGGAGGGGAAGCAGGGAGCCAGCCACACAGGGCCTGCACTTGGGATGAGGGGTGTGGCCTCCAGCTCCAGGTCCTGCCATTTTCTTCTCCTTTTTATGTTTCACCATCTTGGCCCTTTAGTTTCTTAAAGGTGAAAAGCCTTCCCAAAGGGGTTCCCACTTCCTGGAATTTTCTTGCTCTCCTGTCTGGCTCACACCCAGCATCCCTTTTTCCTGAAGGCCTTTCCTATCTCCCCCTCAGGGTGGTCCTCCTGCTTTACACAGCCTCAGTCAGGATGCCTCCTTCTCAGCTCTCTATCTGTATGCTAAATAGATGGATGGATGAATGGATGAGTGAACTGATGGATGGGTGGATGGGTGGACAAATGGATGGGTGGACAGGTGGATGGGTGGACGGGGGATGGGTGGATAGAGGACAGGTGGATGGGTGGACGGGTGGATGGGTGAACAGGTGGATGGGTGGACAAATAGATGGGTGGATGGGTGGACAAGTGGACAAATGGATGGGTGGACAGGTAGACAGGTGGACAAATGGATGAGTGGACAGGTGGACAGGTGGGTGAGTGGACAGGTGGACATGAGGGGGGGTGGACAGGTGGACAAATGAATGGGTGGACCAGTGGACAGGTGGACGGGTGGACAGGTGGATGGGTGGGTGGCTGGGTGGACAGGTGGAAAAAATGGATGGGGGGATGGCTGGACAGGTGGATGGGTGGGTGGATGAACAGGTGGACAAATGGGTGGGTGGACAAGTGGATGTGTAGATGGGTGGATGAATGGATAGGTGAACAGGCAGATGAATAGACAGGTGGATGGGCAAATGAATGGGTGGACAGGGGGTTCATTTACTTGATTTCTGCCTTCCTGACTGGACTGGAAGCTCTGTGAGGGGAGGGGCCCTTCTAGTCCACCTCTGCATCTCCAGAGCTTGAAATCGGTGGGCTGATTTCTGCTGATGGACTGACTGGTGCTGTTGATGGCGTGACTCAAGGAACAAAGGTTCTCAGGTTAGTGCTAGGATCCCAGACTGGACAACAGTCGGGTGAACGCTTCCCCAGGGTCAGTGAAGGAGGAAGCATGATAACGTGTGTAAGATCTGTGACTAGTTCTATAGTTCTATAAACTCTACAAAGCTGCTAATTATTATATAACTGGAAAATATCTATAGCACTTATGCTATACCAACAAATTATGTGCATTTCTACAATTGCCACTATAAAGAAATTATATATTAATACCTGCTCCTGGGACTCAATAGTATGATGGCTTTGAAATTAATACAGCAGCACAAATATCTCCTCAGAAAGACCTTTCCTTGCCCTCATCAAAATCGTCCTTCCCACAGTGCCATCTCATTACTGTTTCGCTTTCTCTCTGGCAATTACTCCTGCTGAAAATGACCATATTTACTTCTTTGCTCGTGAGTTCCGGGATGGCCAGTCCGCCTGGCCTGGAGTGCGGCTTCCCAGGTAGACACAGGAAGGAGTCACCTGGATTGCTCCTTCCTGAATTCCATCATTGAGAACGCGTTACCGTGGGTGGGAGTCAGGGACTGACAAAGTCAAGTCTTGCCAGTTTATTTCTAATTTATATAAGTGTCAAGAGCCAACATGAGCAACTGAATTATAGGTCAGAAAAAGATAATATCAAGAAAATATCCCCAAGATTAGTTTTAATGGTGTGAGTGAATAAATTAGTGAGCAGAGTAAAATGAAATTATGGGAAGGGACTGGGCACGGTGGCTCACGCTTGTAATCCTAGCACTGTGGGAAGCTGAGGCAGGAGGATCATTTGAGCCCAGGAGTTTGAGACCAGCCTAGACAGCACAGCAAGATCCCATGTCACAAAAGCAACAATTAAATTAAAAATATAAAAAGAAAAGAAATGATGGAAGGGGCTACGTGGCATTAACTACATATTGTTTCATTCTAAAGAACTTAATACAATCAATGTATACCTATATTTAGGAGACATTTCTCACACTCATGCCGTGTTTCAGTGGACACTCTCTCATGACAAGCTTTAAGAAAAAAAAGTCTCATAATAGCCTGAATGTATCTGTATCCAGCATAAACAGAAAAGGCGGAACAGTGATCAGGTTGTCTCTGAATGAGATAGGGCCTTTCCCAGCTTCTGCATGGACGTGCAGCCCAATATACACCAAATTAAGAAAACAGGAATGTGCCCTGGAGACGCGGGGAACAACACCACCCTCACCCTCACCCCAGAGACGTGGGGAACAAAGCCGCCCTCGCCCTCACCCTCACCCCAGAGACGTGGGGAACAACGCCGCCCTCACCCTCACCCTCACCCTCACGGCAGAGACGCGGGGAACAACACCACCTGCACCCTCATGCCTCAGCTAACCCTAAATTAAGAGCTTGCTGTGGAGACTTCAGAATCATTTCACTGTATACAGATTCAATGCTCAGGGGTTTAAATGGCATTATTTTTTCCACATACTGGACCTTACCTATTAATATTCAGGCCAGGAACAGCTTAAAATTGCAGAGAGCAAGCACACTAGCCAATAGCTCAGTGGGGAGCTGCGGGTAAGTAGCTGCCAGTCACCAGACCCAGGCCATTGTGAGCACAAGGGCCCTCACAGCAGCCCGACGGAGCCCCCGTCACTCGCAGAGCTCTGCAGGCTTGGGGGGCTGTGGCGAGGGAGGGGGAGGCCGGACCCAGCACAGGAAGAGGGGAGGTGAGGCGAGGCCGGGATGCCCTAGGCCACGCCAGCAGCAGGGACAAGCCCAGGGCAGGCAGGAGGCCCCGGAAATGCCCTGGGAAGGTACCACCTCCCTTCTCCTCCCCCCAGACCCCACTGCGGAGACCCAGCTTTCTGGGGACGCAAGCAGGGTGGCGCATCACAGACAGCGTGCTAGGAGAGTCAGCATAGCTGGGCCTGTTTATCCCGCTCCGTGGGCCAGGTGTTCTTGTCAGGGCGGCCGGTGGGCACGTGGGCCGGGCTGGTTGATGTGAGGGTGCGCCAAGCCCGCCTTCTCACCCAGGCATCCCCGACATGGGATCTGCCCAGCCTGCCTGGCAGTTTTCACTGGGAACATATTGTCCCCATCGTCGCTTAACTACGACTGGCGGGACCATGGGGCTCTGCCGGCCCAGCCCTTCCAGTGATGACGATGCTCCAATGGCCGTTCTCCGTGTCAAGCACCGGACGGCCCACCCTGCGACTCCGTCTCTGCGAGGCACACGCCATCTCCCGCCGCTCCCACTAGGCAGGGAGGGCCGAGCTGCTCCGGACGTATCTGCTGCCTGAGCTCCCTTGGGGCATAGTGGCCAAAAACAACCCTACACAGAGGTGAGGTCTTATCACAAAGGATAACCAATACCATATTTTTTCTTATGACAGCAATGACAATGACAGTGTACGTTCTACCCACAATGACAGATGTCAGCTGGGCTGCCACGTCTTAAGCAGATGTCATAAAATCAGAAAAGCATGGAGTGGCTGAAAGGGCGAGGGTGGCAGAGATGCTTGGGGCCTTTCTCTGAGCAAAAGATGTGGCCTCGTCCTGGAGAGGTGGGAGGGGAGGAGGCAGGTGTATGGAAAGCAGCCAACTGGCAAGAACAGGGTGGCTGCAGTCCTGGCCAGCAGATCCCGGCCACCAGGAGACTCTGAGCACAGGGCAGACACCGTGTGCAGGATGCCATCTCTCCTGCTGGTGAACATCCGGAGGACCTCTTGGCCCCCAGGGGCTGCACAGATAGGAAATATGCATAGATCTGAAAGGCTTTGGGACACATGTGAGGGACAGGGTCATCATCTGCTACCACAAGCAACAACAATATTCCTTGCCATTGAGTCGGTCTCTTTCTCTAGAAAGTAAATTCTCAATACGGGAAAGATTCCCTCCAGGGTGTTTACGGATGGTTGTGAGGGGACAGCTTCGGGACTTCTTACAGTTGCTCTAGCCTTTGTCCTTTGATTTCTTCACTTATTATAAATATGTCTTGAAAGCCTCGGGCTGGATGAAGAGAGGGGAGGGAAACACAGTCCCATCCTCACAGGATCCTTTTAGAGTCAGGCAGACAGTGTGGCAGTCAGCAGCCTGAGGTGATCATTTTCGCTGAAATCTGAATTAAAATTTCACTCGATTAAACATGCCTTCTTCATTTTGAGTAGCCACATTTGAAGTACTAAAGACCCACCCGGGGCCCGTGGGTGTGGTATTAACCAGGACGGACATGGAACAGGAATATTTCCACCGTCACAGGACGTTCCATCAGACGGCACGATTCCGAGTGTATGTGTGTTTTCAGCCACCAGGAAAACAGGCAGAGAAACCACCATGCACTGTGGATGCTCCCCCACCCCCCACCCCCAAGGCCCCTATGGACCCTCTTTGGGATCACTCTTTACTGCTATGTCCCAACTGAACACACACCTCAACACCTCCCAAATCCACACACCCACATGCGCAGACACACACGTTGCCACAGGAAAAGCATGATGTGGTTTATGTAAACCACCTTCTCATGGCGTTATACCTCACATGTGACTCTGGGAAAATGCACCTGCACTAACAGGACACAGTGATTCTATCGGGGATGAGATGGCCCGCACGGGCTTGAGGGGTTCGGTGCCATATTCCCCTCCTTCCTCCCCCACCCCTGGTACCATTGGTCCTCTTGCTCTGGGGTTGAGTGGGCATCTATGATGTGGTGTGTCTTGTCCTCCTGGAGGATCTGGGTGTGGTGTCAGTGCTAATGTCCTGGTAAGGATCATGTTCATTCTGGAGCCAGAGGCTTCAGTTCAGATCCTGGGTCATCTACCATCTATGTGACCTTGGAAAACTATTTAGCCTCTGTGAACCTCGGTTTCCTTATCTGCTCATCAGGGCTTGTTATTATTTTTATACACACCCCTTAGAGTTCTGATGAGGATTAAGTGAGCTAATATGTACCAGGCAATAGAAGAGCTCCTGGCAAATAGGAGTCATGGAATGCATTTTACTTATTGCTATTTGGGACAAAGACTCAATCTCATATTTTTTATACTCTTCATGTCGCTTGGCAGTTTTACTGAGTCGATGTCAGATTACTTAAAAATAATTTTTACTTAGCCTTAGATATGTTTTATATTTTTATCTAATTTTGTCTCCAATCTGTGTATTAAACTAGCTACCTCCAGCTACTGTCTCTATGTACCTGCCATGCTTCAAACATCCACACTGGTTTCAAATCTTTCCACATGTTTTTCTCTCTACTTGGAAAGACTTTTCAATCCTTACCTGACTTGCAAACTCCTGTACAAGCCTTAAAGCCCAGTTCTAAAATGACTTCCTTGAGAAAATATCACTGATTACCTAGGGCAGAAGGGATAGCTCCTTCTGGAATATTCCTATACATCTGTTCAAGCTGCTTTTAAATTATGTGCATCTTTAGTATCAATTTACATGCAGTTCTCCCCCTCCACTGGGAATCCCTGAACTAAGAATACTATCCATCCCACCGTGGCCACTCAGGAGATGTTTAGAATGATTGATATTGAATATCGAAGTTCAGAGATGGTGCGGCAGAGACCCATGGGTGGCACTGTCATCAGTGAGGTCTGGGAAAGACCAGAAGGCATTGAGCTCCTTGCTGCTGGGTCTCCCAGCTCCTTCTCTAGCCCTGGATTGTCTGCCCTGCTTTCCCATGCAACATCCCATGCAGTTTGTTCATTTTGTGGCTGTCTGCAGACGTTTAGGGATATGCAGAGGGGGCTTTAAGCCCTCAGCAAGGAAGAGAATATAGGTCCAACTGACAAGGGTCATTTGCTGAAAGTGAAGTTAATAAAAATCGGCCTCAGACAGGAGAGACTGACCCCCTGCAGAAGGAGCTCCCTCCAGGCAAAGCCAAGCTGGTTCATTCCTCCAAGCTTCTGTTGGGCGTTTCTGCCTCAGACACTCTGCTTAGCACTGAGGAGACGCTGGCACTAGGACGCAGCCAGCCTCTGCCCTCAAGGAGCTGACAGTCTGTTTCGGAAATCTCCAAGGGTCCTAACCCTCTATCTGCATTTCTGAGGCCCGGAGGTCCTGAGAACAAAGACCTTGCTGTTCATTCGGTGGCTGGTGATGGGGTGTTGCGTGTCTGGGGTCCGCGCCAGCTGACCACTCACTAGGCAGCAGAACCTCGCTGAACTGTGGAGGCTATTTCTAGTCTATACCCCAGTAAATGTGCACGCCTGACATTTTAACATGGAAATATTGGTGTGTTTGATTTTGAAGGGTCGTTCAGGCCCTGTGCAATATTTGTAGCCAATGTATGCATTCTTCTGAAACCTGACAGATTCTGAACACATCTGCCTATGGGGGTTTTGGCCAAAGCCTGTTGGACTTGGAATCAGGTTAAGTTTTAGGAATATCTATCAAGGACACCAGAGTGTGTCCTGAAACCAGTCAAGATGAGAGAGGTGGGCCGGAAGCGATGGCTCACTCCAGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCAGATTACCTGAGATCAGGAGTTAGCCTGGCCAACATGGTGAAACCTAGTTTCTACTAATAATACAAAAATTAGCCAGGCATGGTGGTGCATGCCTGTAATCCCAGCAACTCAGGAGGCTGAGGCAGGCGAATTGCTTGAACCTGGGAGGTGGTGGTTGCAGTGAGCTGGGATCGTGCCACTGCCCTCTACCCCAGGTGACAAGAGCAAAACTCCATCTCAAAAACAAACAAACAAACAAACAAAACCAACCAACCAACCAAACAAACAAACAAACAAAAAAAGAGAGGCAAGATGGCCCCAGGAAACCAAAGGTCATGGTACCATGTAAATAACACAACCACCTGAAGATGGCTGCAGCCCAAGGGACTGTGTTTTCAGACACCATGGCTGCGAGAAGAACAGAGTGGGACAGGAGGGAGACACCACAGCACCCACTCCCCTCCGGGTACCCACTCCCCTCTGGGTACCCACTCCTCTCCGGGCACCCACTCCCCTCCAGGCACCCACTCCCCTCCGGGTACACACTCCTCTCCAGGCACCCACTCCCCTCCGGGCACCCACTCCCCTCAGGGCACCCACTCCCCTCTGGGTACCCACTCCCCTCCGGGTACCCACCTCTCTCCGGGCACCCACTCCCCTCCGGGCACCCACTCCTCTCCAGGCACCCACTCCCCTCAGGGCACCCACTCCCCTCCGGGCACCCACTCCCCTCCAGGACGCTCCAGCGTCTGGCTTCTGGACGTGTCCACTGCTGAGTGTACACAGGCCTCAGCTCCCACCTGGGCAAACATCGCCTGGGCAGTCGTCCCCTGTGCCAGTCCCAGGACCCAGGGCCCTGTGTGCTGCTCCTCACAGGCGACCTTCTGCCTACCGCCTGTGAACAGCCAAACCAGCTGGCTGGAGGCTGGGAGAACAGGGCCCCGAGGCTGAGTCAGCCTGGGGTCCCAGCCAAGATGCAGATGCGGAGAGACCCAGGCCCCCCGCAGCTGACCAGACACGTGAGAGGGGCAGCCGGGCCCAGCGGACCCTGAGCCCATCCCAGGCCACACTGCCCAGGCACAGAGCGTGGAGCTACATAAACAGGCGTTGCTTTAAGCCCCTAAGGTGTGGAGCAGCAGTGCAGTTGGAGGCACCCTGTGTGTGCTCACAGATACAGGCGGAATCAGGCAAGGGCTGCTGGGAAAGAACGGCAGGGCCACATCGGCCTCTGCGGGACAAGGACACCGGCTCCCGATGCCTGAAAGTGCCTGCACCAGCCTCACCGTGCACTCTGCCTCCAAGAGCAGAGAGTGTCTCGCAACCACATCGCTTCCTGGCTACGCCGTTCTGCAGGCTGAAATGGCTTCCATTCACCCAAGCACAATTCCTGGAGAATCCTGAGACACACGAGGCTCACGTTTTCTCTATGTATCTAATTACCAGGAATAATGAGGCAGGGAAACCTGAACGGTTGTGAATTATCCCACAAGAAGACGTCCACGGGCAGAGGCCACCTGTCGCAGGGGAAGGCAGGCAGCTGTCCCTGGGAATGACACACAGCCCACCTTCTTTCCCTCGGCTCCTCGGTGTGTTAATCTGATGTGTCTGTTTCCCTCTAAAGGTTTTGTTCTTTTCACACTGCAGATGGATGTGCAATATGCCGCTTTTGCACTAGAGGCAAGAGAATCAGGTGGAATCTCACAGCACTTCTCCCGGACTCCTGGGCCGTCTGCACCACGTCCAAGTCAAGGGCCCTGGACTGAGGCACATGCCCACTTCCTGCGTCTTTGCGATAAAATGAGTGTGCGGTTCACACTCACTTCCTGCATCCCTGAGGTGAGGCTGTGGTGGTGAGGCCGTGGTGGTGAGGCCGTGTGGTTGAGGCCGTGGGGGTGAGGCCGTGGGGTGAGGCCATGGGGTTGAGGCTGTGGGGTGAGGCCGG
     READ_BASE_COUNTS: 6282, 6408, 6286, 6163, 0
          READ_FILTER: SRA_READ_FILTER_PASS
             READ_LEN: 25139
           READ_START: 0
            READ_TYPE: SRA_READ_TYPE_BIOLOGICAL
          REPLACED_BY:
             REPLACES:
     SEQID_GNL_PREFIX: WGS:JTFH01
               SEQ_ID: gi|725026265|gb|JTFH01000001.1|
           SEQ_ID_GNL: gnl|WGS:JTFH01|decoy00001
           SPOT_COUNT: 1998
           SPOT_GROUP:
              SPOT_ID: 1
             SPOT_LEN: 25139
               STRAIN:
    TARGETED_LOCUS_ID:
  TARGETED_LOCUS_NAME:
                TAXID: 9606
                TITLE: Homo sapiens isolate Pool of 254 public SGDP samples decoy00001, whole genome shotgun sequence
             TRIM_LEN: 25139
           TRIM_START: 0

@klymenko
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Run vdb-config -i;
Uncheck "Enable remote access", if it is enabled (type 'r');
type 's' to save;
type 'x' to exit.

Then run again align-info path/SRR22287940 and post here the output.

@sa2adeel
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sa2adeel commented Jul 13, 2024 •
edited
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JTFH01000001,chrUn_JTFH01000001v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000002,chrUn_JTFH01000002v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000003,chrUn_JTFH01000003v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000004,chrUn_JTFH01000004v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000007,chrUn_JTFH01000007v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000008,chrUn_JTFH01000008v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000012,chrUn_JTFH01000012v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000013,chrUn_JTFH01000013v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000015,chrUn_JTFH01000015v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000017,chrUn_JTFH01000017v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000018,chrUn_JTFH01000018v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000022,chrUn_JTFH01000022v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000025,chrUn_JTFH01000025v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000026,chrUn_JTFH01000026v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000029,chrUn_JTFH01000029v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000031,chrUn_JTFH01000031v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000033,chrUn_JTFH01000033v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000039,chrUn_JTFH01000039v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000040,chrUn_JTFH01000040v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000044,chrUn_JTFH01000044v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000045,chrUn_JTFH01000045v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000050,chrUn_JTFH01000050v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000054,chrUn_JTFH01000054v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000059,chrUn_JTFH01000059v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000060,chrUn_JTFH01000060v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000061,chrUn_JTFH01000061v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000063,chrUn_JTFH01000063v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000064,chrUn_JTFH01000064v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000068,chrUn_JTFH01000068v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000070,chrUn_JTFH01000070v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000071,chrUn_JTFH01000071v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000072,chrUn_JTFH01000072v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000077,chrUn_JTFH01000077v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000079,chrUn_JTFH01000079v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000087,chrUn_JTFH01000087v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000089,chrUn_JTFH01000089v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000090,chrUn_JTFH01000090v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000092,chrUn_JTFH01000092v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000096,chrUn_JTFH01000096v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000098,chrUn_JTFH01000098v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000101,chrUn_JTFH01000101v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000107,chrUn_JTFH01000107v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000108,chrUn_JTFH01000108v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000110,chrUn_JTFH01000110v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000111,chrUn_JTFH01000111v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000112,chrUn_JTFH01000112v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000113,chrUn_JTFH01000113v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000114,chrUn_JTFH01000114v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000116,chrUn_JTFH01000116v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000117,chrUn_JTFH01000117v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000118,chrUn_JTFH01000118v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000119,chrUn_JTFH01000119v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000123,chrUn_JTFH01000123v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000124,chrUn_JTFH01000124v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000126,chrUn_JTFH01000126v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000127,chrUn_JTFH01000127v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000129,chrUn_JTFH01000129v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000130,chrUn_JTFH01000130v1_decoy,false,remote:path/SRR22287940/JTFH01
JTFH01000132,chrUn_JTFH01000132v1_decoy,false,remote:path/SRR22287940/JTFH01

@klymenko
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Try to run fasterq-dump or fastq-dump of /cluster/projects/hopegroup/dbGap/prj_35579/SRR22287940 again.
Does it fail now?

@sa2adeel
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sa2adeel commented Jul 13, 2024 •
edited
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Still fails with fasterq-dump.

2024-07-13T18:58:29 fasterq-dump.3.1.1 warn: directory not found while opening manager within virtual file system module - can't open JTFH01000001 as a RefSeq or as a WGS
2024-07-13T18:58:29 fasterq-dump.3.1.1 err: sorter.c producer_thread_func: rec.read.len = 0
2024-07-13T18:58:30 fasterq-dump.3.1.1 err: sorter.c run_producer_pool().join_and_release_threads -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)
2024-07-13T18:58:30 fasterq-dump.3.1.1 err: sorter.c execute_lookup_production() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)
2024-07-13T18:58:30 fasterq-dump.3.1.1 err: fasterq-dump.c produce_lookup_files() -> RC(rcVDB,rcNoTarg,rcWriting,rcFormat,rcNull)

=============================================================
An error occurred during processing.
A report was generated into the file 'usr/ncbi_error_report.txt'.
If the problem persists, you may consider sending the file
to '[email protected]' for assistance.
=============================================================

fasterq-dump quit with error code 3

@klymenko
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I will need to debug it on Monday.

@klymenko
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What ngc file did you use to prefetch SRR22287940?

@sa2adeel
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The ngc file was downloaded from the dbGap repository that is specific to our groups data request application. It was the same file used to download the other accessions. I am not sure if I can share the file.

@klymenko
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Don't share it. What is project's number?

@sa2adeel
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project # 35579

@klymenko
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How many files do you have in SRR22287940/ directory?

@sa2adeel
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There are 592 files in total.

@klymenko
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What is the output of the following?
align-info /cluster/projects/hopegroup/dbGap/prj_35579/SRR22287940 | wc -l

@sa2adeel
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I get 1552 back.

@klymenko
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What is the output of the following?
ls -l /cluster/projects/hopegroup/dbGap/prj_35579/SRR22287940/SRR22287940*

@sa2adeel
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-rw-r----- 1 usr gdc 1278275591 Jul 8 16:05 SRR22287940/SRR22287940.sra

@klymenko
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It is a bug, thank you for finding it, we will fix it in our following release.

Currently run fasterq-dump from the same directory where run directory SRR22287940/ is located (/cluster/projects/hopegroup/dbGap/prj_35579).

Use --outdir argument to specify directory where to write output files.

@sa2adeel
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Awesome, thank you!

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