diff --git a/csharp/test/Apache.Arrow.Tests/CDataInterfacePythonTests.cs b/csharp/test/Apache.Arrow.Tests/CDataInterfacePythonTests.cs
index 4c53b98e3d9f1..86d7ff52cdf09 100644
--- a/csharp/test/Apache.Arrow.Tests/CDataInterfacePythonTests.cs
+++ b/csharp/test/Apache.Arrow.Tests/CDataInterfacePythonTests.cs
@@ -60,7 +60,9 @@ public PythonNet()
 
             public void Dispose()
             {
+#if !NET5_0_OR_GREATER
                 PythonEngine.Shutdown();
+#endif
             }
         }