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gene-therapy.mdwn

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Here are some gene therapy delivery methods.

naked DNA

hydrodynamic injection

viruses and viral gene therapy

electroporation

gene gun

sonoporation

gas-filled micro-bubbles

magnetofection and magnetic nanoparticles

  • magnetic calcium phosphate nano-formulations

carbon nanotubes

quantum dots

  • super paramagnetic iron oxide nanoparticles (SPIONS)

lipoplexes

dendrimers

inorganic nanoparticles

  • carbon nanotuubes
  • magnetic nanoparticles (see also magnetofection)
  • calcium phosphate nanoparticles
  • gold nanoparticles
  • quantum dots
  • C60 fullerenes
  • aminofullerenes
  • silica nanoparticles

gold nanoparticles

cell-penetrating peptides

polymerosomes

liposomes

polyplexes (DNA + plastic)

  • polyion complex micelles (PICs)

cationic polymers

  • collagen
  • albumin
  • beta-casein
  • zein

cationic lipids

  • dioleylpropyl trimethylammonium chloride (DOTMA)
  • dioleoyl trimethylammonium propane (DOTAP)

cationic emulsions

solid lipid nanoparticles

virosomes

protofection

natural competence

Ligandal's nano-gobstopper method

(patent) Nanoparticle-mediated gene delivery, genomic editing and ligand-targeted modification in various cell populations

conjugation of PEI for DNA binding, histone tail peptide for nuclear localization, poly-glutamic acid, glutamic acid for stabilization and charge balancing or whatever, then you stick DNA to it, and silica coat the whole thing, then add even more cell-targeting stuff to the outside.

other

  • poly lactic-co-glycolic acid (PLGA)-based nanoparticles
  • hybrid nanoparticles such as liposome-polycation-DNA (LPD) nanoparticles
  • lipopolyplexes

hplusroadmap logs

See http://gnusha.org/logs/2017-01-09.log for some preliminary stuff.