-
Notifications
You must be signed in to change notification settings - Fork 0
/
bwa-postalt.js
633 lines (589 loc) · 21.5 KB
/
bwa-postalt.js
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
/*****************************************************************
* The K8 Javascript interpreter is required to run this script. *
* *
* Source code: https://github.com/attractivechaos/k8 *
* Binary: http://sourceforge.net/projects/lh3/files/k8/ *
* *
* Data file used for generating GRCh38 ALT alignments: *
* *
* http://sourceforge.net/projects/bio-bwa/files/ *
*****************************************************************/
/******************
*** From k8.js ***
******************/
// Parse command-line options. A BSD getopt() clone in javascript.
var getopt = function(args, ostr) {
var oli; // option letter list index
if (typeof(getopt.place) == 'undefined')
getopt.ind = 0, getopt.arg = null, getopt.place = -1;
if (getopt.place == -1) { // update scanning pointer
if (getopt.ind >= args.length || args[getopt.ind].charAt(getopt.place = 0) != '-') {
getopt.place = -1;
return null;
}
if (getopt.place + 1 < args[getopt.ind].length && args[getopt.ind].charAt(++getopt.place) == '-') { // found "--"
++getopt.ind;
getopt.place = -1;
return null;
}
}
var optopt = args[getopt.ind].charAt(getopt.place++); // character checked for validity
if (optopt == ':' || (oli = ostr.indexOf(optopt)) < 0) {
if (optopt == '-') return null; // if the user didn't specify '-' as an option, assume it means null.
if (getopt.place < 0) ++getopt.ind;
return '?';
}
if (oli+1 >= ostr.length || ostr.charAt(++oli) != ':') { // don't need argument
getopt.arg = null;
if (getopt.place < 0 || getopt.place >= args[getopt.ind].length) ++getopt.ind, getopt.place = -1;
} else { // need an argument
if (getopt.place >= 0 && getopt.place < args[getopt.ind].length)
getopt.arg = args[getopt.ind].substr(getopt.place);
else if (args.length <= ++getopt.ind) { // no arg
getopt.place = -1;
if (ostr.length > 0 && ostr.charAt(0) == ':') return ':';
return '?';
} else getopt.arg = args[getopt.ind]; // white space
getopt.place = -1;
++getopt.ind;
}
return optopt;
}
// print an object in a format similar to JSON. For debugging only.
function obj2str(o)
{
if (typeof(o) != 'object') {
return o.toString();
} else if (o == null) {
return "null";
} else if (Array.isArray(o)) {
var s = "[";
for (var i = 0; i < o.length; ++i) {
if (i) s += ',';
s += obj2str(o[i]);
}
return s + "]";
} else {
var i = 0, s = "{";
for (var key in o) {
if (i++) s += ',';
s += key + ":";
s += obj2str(o[key]);
}
return s + "}";
}
}
// reverse a string
Bytes.prototype.reverse = function()
{
for (var i = 0; i < this.length>>1; ++i) {
var tmp = this[i];
this[i] = this[this.length - i - 1];
this[this.length - i - 1] = tmp;
}
}
// reverse complement a DNA string
Bytes.prototype.revcomp = function()
{
if (Bytes.rctab == null) {
var s1 = 'WSATUGCYRKMBDHVNwsatugcyrkmbdhvn';
var s2 = 'WSTAACGRYMKVHDBNwstaacgrymkvhdbn';
Bytes.rctab = [];
for (var i = 0; i < 256; ++i) Bytes.rctab[i] = 0;
for (var i = 0; i < s1.length; ++i)
Bytes.rctab[s1.charCodeAt(i)] = s2.charCodeAt(i);
}
for (var i = 0; i < this.length>>1; ++i) {
var tmp = this[this.length - i - 1];
this[this.length - i - 1] = Bytes.rctab[this[i]];
this[i] = Bytes.rctab[tmp];
}
if (this.length>>1)
this[this.length>>1] = Bytes.rctab[this[this.length>>1]];
}
// create index for a list of intervals for fast interval queries; ported from bedidx.c in samtools
function intv_ovlp(intv, bits)
{
if (typeof bits == "undefined") bits = 13;
intv.sort(function(a,b) {return a[0]-b[0];});
// create the index
var idx = [], max = 0;
for (var i = 0; i < intv.length; ++i) {
var b = intv[i][0]>>bits;
var e = (intv[i][1]-1)>>bits;
if (b != e) {
for (var j = b; j <= e; ++j)
if (idx[j] == null) idx[j] = i;
} else if (idx[b] == null) idx[b] = i;
max = max > e? max : e;
}
// closure
return function(_b, _e) {
var x = _b >> bits;
if (x > max) return [];
var off = idx[x];
if (off == null) {
var i;
for (i = ((_e - 1) >> bits) - 1; i >= 0; --i)
if (idx[i] != null) break;
off = i < 0? 0 : idx[i];
}
var ovlp = [];
for (var i = off; i < intv.length && intv[i][0] < _e; ++i)
if (intv[i][1] > _b) ovlp.push(intv[i]);
return ovlp;
}
}
var re_cigar = /(\d+)([MIDSHN])/g;
/******************************
*** Generate ALT alignment ***
******************************/
// given a pos on ALT and the ALT-to-REF CIGAR, find the pos on REF
function cigar2pos(cigar, pos)
{
var x = 0, y = 0;
for (var i = 0; i < cigar.length; ++i) {
var op = cigar[i][0], len = cigar[i][1];
if (op == 'M') {
if (y <= pos && pos < y + len)
return x + (pos - y);
x += len, y += len;
} else if (op == 'D') {
x += len;
} else if (op == 'I') {
if (y <= pos && pos < y + len)
return x;
y += len;
} else if (op == 'S' || op == 'H') {
if (y <= pos && pos < y + len)
return -1;
y += len;
}
}
return -1;
}
// Parse a hit. $s is an array that looks something like ["chr1", "+12345", "100M", 5]
// Return an object keeping various information about the alignment.
function parse_hit(s, opt)
{
var h = {};
h.ctg = s[0];
h.start = parseInt(s[1].substr(1)) - 1;
h.rev = (s[1].charAt(0) == '-');
h.cigar = s[2];
h.NM = parseInt(s[3]);
h.hard = false;
var m, l_ins, n_ins, l_del, n_del, l_match, l_skip, l_clip;
l_ins = l_del = n_ins = n_del = l_match = l_skip = l_clip = 0;
while ((m = re_cigar.exec(h.cigar)) != null) {
var l = parseInt(m[1]);
if (m[2] == 'M') l_match += l;
else if (m[2] == 'D') ++n_del, l_del += l;
else if (m[2] == 'I') ++n_ins, l_ins += l;
else if (m[2] == 'N') l_skip += l;
else if (m[2] == 'H' || m[2] == 'S') {
l_clip += l;
if (m[2] == 'H') h.hard = true;
}
}
h.end = h.start + l_match + l_del + l_skip;
h.NM = h.NM > l_del + l_ins? h.NM : l_del + l_ins;
h.score = Math.floor((opt.a * l_match - (opt.a + opt.b) * (h.NM - l_del - l_ins) - opt.o * (n_del + n_ins) - opt.e * (l_del + l_ins)) / opt.a + .499);
h.l_query = l_match + l_ins + l_clip;
return h;
}
function print_buffer(buf2, fp_hla, hla)
{
if (buf2.length == 0) return;
for (var i = 0; i < buf2.length; ++i)
print(buf2[i].join("\t"));
if (fp_hla != null) {
var name = buf2[0][0] + '/' + (buf2[0][1]>>6&3) + ((buf2[0][1]&16)? '-' : '+');
for (var x in hla) {
if (fp_hla[x] != null);
fp_hla[x].write('@' + name + '\n' + buf2[0][9] + '\n+\n' + buf2[0][10] + '\n');
}
}
}
function bwa_postalt(args)
{
var c, opt = { a:1, b:4, o:6, e:1, verbose:3, show_pri:false, update_mapq:true, min_mapq:10, min_sc:90, max_nm_sc:10, show_ev:false, min_pa_ratio:1 };
while ((c = getopt(args, 'Pqev:p:r:')) != null) {
if (c == 'v') opt.verbose = parseInt(getopt.arg);
else if (c == 'p') opt.pre = getopt.arg;
else if (c == 'P') opt.show_pri = true;
else if (c == 'q') opt.recover_maq = false;
else if (c == 'e') opt.show_ev = true;
else if (c == 'r') opt.min_pa_ratio = parseFloat(getopt.arg);
}
if (opt.min_pa_ratio > 1.) opt.min_pa_ratio = 1.;
if (opt.show_ev && opt.pre == null) {
warn("ERROR: option '-p' must be specified if '-e' is applied.");
exit(1);
}
if (args.length == getopt.ind) {
print("");
print("Usage: k8 bwa-postalt.js [options] <alt.sam> [aln.sam]\n");
print("Options: -p STR prefix of file(s) for additional information [null]");
print(" PREFIX.ctw - weight of each ALT contig");
print(" PREFIX.evi - reads supporting ALT contigs (effective with -e)");
print(" -e show reads supporting ALT contigs into file PREFIX.evi");
print(" -r FLOAT reduce mapQ to 0 if not overlapping lifted best and pa<FLOAT ["+opt.min_pa_ratio+"]");
print(" -q don't modify mapQ for non-ALTs hit overlapping lifted ALT");
print("");
print("Note: This script extracts the XA tag, lifts the mapping positions of ALT hits to");
print(" the primary assembly, groups them and then estimates mapQ across groups. If");
print(" a non-ALT hit overlaps a lifted ALT hit, its mapping quality is set to the");
print(" smaller between its original mapQ and the adjusted mapQ of the ALT hit. If");
print(" multiple ALT hits are lifted to the same position, they will yield new SAM");
print(" lines with the same mapQ.");
print("");
exit(1);
}
var fp_evi = opt.show_ev && opt.pre? new File(opt.pre + '.evi', "w") : null;
var aux = new Bytes(); // used for reverse and reverse complement
var buf = new Bytes();
// read ALT-to-REF alignment
var intv_alt = {}, intv_pri = {}, idx_un = {}, hla_ctg = {};
var file = new File(args[getopt.ind]);
while (file.readline(buf) >= 0) {
var line = buf.toString();
if (line.charAt(0) == '@') continue;
var t = line.split("\t");
if (t.length < 11) continue; // incomplete lines
var pos = parseInt(t[3]) - 1;
var flag = parseInt(t[1]);
if ((flag&4) || t[2] == '*') {
idx_un[t[0]] = true;
continue;
}
var m, cigar = [], l_qaln = 0, l_tlen = 0, l_qclip = 0;
if ((m = /^(HLA-[^\s\*]+)\*\d+/.exec(t[0])) != null) { // read HLA contigs
if (hla_ctg[m[1]] == null) hla_ctg[m[1]] = 0;
++hla_ctg[m[1]];
}
while ((m = re_cigar.exec(t[5])) != null) {
var l = parseInt(m[1]);
cigar.push([m[2] != 'H'? m[2] : 'S', l]); // convert hard clip to soft clip
if (m[2] == 'M') l_qaln += l, l_tlen += l;
else if (m[2] == 'I') l_qaln += l;
else if (m[2] == 'S' || m[2] == 'H') l_qclip += l;
else if (m[2] == 'D' || m[2] == 'N') l_tlen += l;
}
var j = flag&16? cigar.length-1 : 0;
var start = cigar[j][0] == 'S'? cigar[j][1] : 0;
if (intv_alt[t[0]] == null) intv_alt[t[0]] = [];
intv_alt[t[0]].push([start, start + l_qaln, l_qaln + l_qclip, t[2], flag&16? true : false, pos - 1, cigar, pos + l_tlen]);
if (intv_pri[t[2]] == null) intv_pri[t[2]] = [];
intv_pri[t[2]].push([pos, pos + l_tlen, t[0]]);
}
file.close();
var idx_alt = {}, idx_pri = {};
for (var ctg in intv_alt)
idx_alt[ctg] = intv_ovlp(intv_alt[ctg]);
for (var ctg in intv_pri)
idx_pri[ctg] = intv_ovlp(intv_pri[ctg]);
// initialize the list of HLA contigs
var fp_hla = null;
if (opt.pre) {
fp_hla = {};
for (var h in hla_ctg)
fp_hla[h] = new File(opt.pre + '.' + h + '.fq', "w");
}
// initialize the list of ALT contigs
var weight_alt = [];
for (var ctg in idx_alt)
weight_alt[ctg] = [0, 0, 0, 0, 0, 0, intv_alt[ctg][0][3], intv_alt[ctg][0][5], intv_alt[ctg][0][7]];
for (var ctg in idx_un)
weight_alt[ctg] = [0, 0, 0, 0, 0, 0, '~', 0, 0];
// process SAM
var buf2 = [], hla = {};
file = args.length - getopt.ind >= 2? new File(args[getopt.ind+1]) : new File();
while (file.readline(buf) >= 0) {
var m, line = buf.toString();
if (line.charAt(0) == '@') { // print and then skip the header line
print(line);
continue;
}
var t = line.split("\t");
t[1] = parseInt(t[1]); t[3] = parseInt(t[3]); t[4] = parseInt(t[4]);
// print bufferred reads
if (buf2.length && (buf2[0][0] != t[0] || (buf2[0][1]&0xc0) != (t[1]&0xc0))) {
print_buffer(buf2, fp_hla, hla);
buf2 = [], hla = {};
}
// skip unmapped lines
if (t[1]&4) {
buf2.push(t);
continue;
}
// parse the reported hit
var NM = (m = /\tNM:i:(\d+)/.exec(line)) == null? '0' : m[1];
var flag = t[1];
var h = parse_hit([t[2], ((flag&16)?'-':'+') + t[3], t[5], NM], opt);
if (h.hard) { // the following does not work with hard clipped alignments
buf2.push(t);
continue;
}
var hits = [h];
// parse hits in the XA tag
if ((m = /\tXA:Z:(\S+)/.exec(line)) != null) {
var XA_strs = m[1].split(";");
for (var i = 0; i < XA_strs.length; ++i)
if (XA_strs[i] != '') // as the last symbol in an XA tag is ";", the last split is an empty string
hits.push(parse_hit(XA_strs[i].split(","), opt));
}
// check if there are ALT hits
var has_alt = false;
for (var i = 0; i < hits.length; ++i)
if (weight_alt[hits[i].ctg] != null) {
has_alt = true;
break;
}
if (!has_alt) {
buf2.push(t);
continue;
}
// lift mapping positions to the primary assembly
var n_rpt_lifted = 0, rpt_lifted = null;
for (var i = 0; i < hits.length; ++i) {
var a, h = hits[i];
if (idx_alt[h.ctg] == null || (a = idx_alt[h.ctg](h.start, h.end)) == null || a.length == 0)
continue;
// find the approximate position on the primary assembly
var lifted = [];
for (var j = 0; j < a.length; ++j) {
var s, e;
if (!a[j][4]) { // ALT is mapped to the forward strand of the primary assembly
s = cigar2pos(a[j][6], h.start);
e = cigar2pos(a[j][6], h.end - 1) + 1;
} else {
s = cigar2pos(a[j][6], a[j][2] - h.end);
e = cigar2pos(a[j][6], a[j][2] - h.start - 1) + 1;
}
if (s < 0 || e < 0) continue; // read is mapped to clippings in the ALT-to-chr alignment
s += a[j][5]; e += a[j][5];
lifted.push([a[j][3], (h.rev!=a[j][4]), s, e]);
if (i == 0) ++n_rpt_lifted;
}
if (i == 0 && n_rpt_lifted == 1) rpt_lifted = lifted[0].slice(0);
if (lifted.length) hits[i].lifted = lifted;
}
// prepare for hits grouping
for (var i = 0; i < hits.length; ++i) { // set keys for sorting
if (hits[i].lifted != null) // TODO: only the first element in lifted[] is used
hits[i].pctg = hits[i].lifted[0][0], hits[i].pstart = hits[i].lifted[0][2], hits[i].pend = hits[i].lifted[0][3];
else hits[i].pctg = hits[i].ctg, hits[i].pstart = hits[i].start, hits[i].pend = hits[i].end;
hits[i].i = i; // keep the original index
}
// group hits based on the lifted positions on non-ALT sequences
if (hits.length > 1) {
hits.sort(function(a,b) { return a.pctg != b.pctg? (a.pctg < b.pctg? -1 : 1) : a.pstart - b.pstart });
var last_chr = null, end = 0, g = -1;
for (var i = 0; i < hits.length; ++i) {
if (last_chr != hits[i].pctg) ++g, last_chr = hits[i].pctg, end = 0;
else if (hits[i].pstart >= end) ++g;
hits[i].g = g;
end = end > hits[i].pend? end : hits[i].pend;
}
} else hits[0].g = 0;
// find the index and group id of the reported hit; find the size of the reported group
var reported_g = null, reported_i = null, n_group0 = 0;
if (hits.length > 1) {
for (var i = 0; i < hits.length; ++i)
if (hits[i].i == 0)
reported_g = hits[i].g, reported_i = i;
for (var i = 0; i < hits.length; ++i)
if (hits[i].g == reported_g)
++n_group0;
} else {
if (weight_alt[hits[0].ctg] == null) { // no need to go through the following if the single hit is non-ALT
buf2.push(t);
continue;
}
reported_g = reported_i = 0, n_group0 = 1;
}
// re-estimate mapping quality if necessary
var mapQ, ori_mapQ = t[4];
if (n_group0 > 1) {
var group_max = [];
for (var i = 0; i < hits.length; ++i) {
var g = hits[i].g;
if (group_max[g] == null || group_max[g][0] < hits[i].score)
group_max[g] = [hits[i].score, g];
}
if (group_max.length > 1)
group_max.sort(function(x,y) {return y[0]-x[0]});
if (group_max[0][1] == reported_g) { // the best hit is the hit reported in SAM
mapQ = group_max.length == 1? 60 : 6 * (group_max[0][0] - group_max[1][0]);
} else mapQ = 0;
mapQ = mapQ < 60? mapQ : 60;
if (idx_alt[t[2]] == null) mapQ = mapQ < ori_mapQ? mapQ : ori_mapQ;
else mapQ = mapQ > ori_mapQ? mapQ : ori_mapQ;
} else mapQ = t[4];
var pri_ofunc = idx_pri[hits[reported_i].pctg], ovlp_alt = [];
if (pri_ofunc != null) {
var rpt_start = 1<<30, rpt_end = 0;
for (var i = 0; i < hits.length; ++i) {
var h = hits[i];
if (h.g == reported_g) {
rpt_start = rpt_start < h.pstart? rpt_start : h.pstart;
rpt_end = rpt_end > h.pend ? rpt_end : h.pend;
}
}
ovlp_alt = pri_ofunc(rpt_start, rpt_end);
for (var i = 0; i < ovlp_alt.length; ++i)
if ((m = /^(HLA-[^\s\*]+)\*\d+/.exec(ovlp_alt[i][2])) != null)
hla[m[1]] = true;
}
// ALT genotyping
if (mapQ >= opt.min_mapq && hits[reported_i].score >= opt.min_sc) {
// collect all overlapping ALT contigs
var alts = {};
for (var i = 0; i < hits.length; ++i) {
var h = hits[i];
if (h.g == reported_g && weight_alt[h.ctg] != null)
alts[h.ctg] = [h.score, h.NM];
}
if (ovlp_alt.length > 0) { // add other unreported hits
for (var i = 0; i < ovlp_alt.length; ++i)
if (ovlp_alt[i][0] <= rpt_start && rpt_end <= ovlp_alt[i][1] && alts[ovlp_alt[i][2]] == null)
alts[ovlp_alt[i][2]] = [0, 0];
}
// add weight to each ALT contig
var alt_arr = [], max_sc = -1, max_i = -1, sum = 0, min_sc = 1<<30, max_nm = -1;
for (var ctg in alts)
alt_arr.push([ctg, alts[ctg][0], 0, alts[ctg][1]]);
for (var i = 0; i < alt_arr.length; ++i) {
if (max_sc < alt_arr[i][1])
max_sc = alt_arr[i][1], max_i = i;
min_sc = min_sc < alt_arr[i][1]? min_sc : alt_arr[i][1];
var nm = alt_arr[i][1] > 0? alt_arr[i][3] : opt.max_nm_sc;
max_nm = max_nm > nm? max_nm : nm;
}
if (max_nm > opt.max_nm_sc) max_nm = opt.max_nm_sc;
if (max_sc > 0 && (alt_arr.length == 1 || min_sc < max_sc)) {
for (var i = 0; i < alt_arr.length; ++i)
sum += (alt_arr[i][2] = Math.pow(10, .6 * (alt_arr[i][1] - max_sc)));
for (var i = 0; i < alt_arr.length; ++i) alt_arr[i][2] /= sum;
for (var i = 0; i < alt_arr.length; ++i) {
var e = [alt_arr[i][0], 1, alt_arr[max_i][2], alt_arr[i][2], max_nm, max_nm * alt_arr[max_i][2], max_nm * alt_arr[i][2]];
var w = weight_alt[e[0]];
for (var j = 0; j < 6; ++j) w[j] += e[j+1];
if (fp_evi) {
e[2] = e[2].toFixed(3); e[3] = e[3].toFixed(3);
e[5] = e[5].toFixed(3); e[6] = e[6].toFixed(3);
fp_evi.write(t[0] + '/' + (t[1]>>6&3) + '\t' + e.join("\t") + '\n');
}
}
}
}
// check if the reported hit overlaps a hit to the primary assembly; if so, don't reduce mapping quality
if (opt.update_mapq && mapQ > 0 && n_rpt_lifted <= 1) {
var l = n_rpt_lifted == 1? rpt_lifted : null;
for (var i = 0; i < buf2.length; ++i) {
var s = buf2[i], is_ovlp = true;
if (l != null) {
if (l[0] != s[2]) is_ovlp = false; // different chr
if (((s[1]&16) != 0) != l[1]) is_ovlp = false; // different strand
var start = s[3] - 1, end = start;
while ((m = re_cigar.exec(t[5])) != null)
if (m[2] == 'M' || m[2] == 'D' || m[2] == 'N')
end += parseInt(m[1]);
if (!(start < l[3] && l[2] < end)) is_ovlp = false; // no overlap
} else is_ovlp = false;
var om = -1, pa = 10.;
for (var j = 11; j < s.length; ++j)
if ((m = /^om:i:(\d+)/.exec(s[j])) != null)
om = parseInt(m[1]);
else if ((m = /^pa:f:(\S+)/.exec(s[j])) != null)
pa = parseFloat(m[1]);
if (is_ovlp) { // overlapping the lifted hit
if (om > 0) s[4] = om;
s[4] = s[4] < mapQ? s[4] : mapQ;
} else if (pa < opt.min_pa_ratio) { // not overlapping; has a small pa
if (om < 0) s.push("om:i:" + s[4]);
s[4] = 0;
}
}
}
// generate lifted_str
for (var i = 0; i < hits.length; ++i) {
if (hits[i].lifted && hits[i].lifted.length) {
var u = '', lifted = hits[i].lifted;
for (var j = 0; j < lifted.length; ++j)
u += lifted[j][0] + "," + lifted[j][2] + "," + lifted[j][3] + "," + (lifted[j][1]?'-':'+') + ";";
hits[i].lifted_str = u;
}
}
// generate reversed quality and reverse-complemented sequence if necessary
var rs = null, rq = null; // reversed quality and reverse complement sequence
var need_rev = false;
for (var i = 0; i < hits.length; ++i) {
if (hits[i].g != reported_g || i == reported_i) continue;
if (hits[i].rev != hits[reported_i].rev)
need_rev = true;
}
if (need_rev) { // reverse and reverse complement
aux.length = 0;
aux.set(t[9], 0); aux.revcomp(); rs = aux.toString();
aux.set(t[10],0); aux.reverse(); rq = aux.toString();
}
// stage the reported hit
t[4] = mapQ;
if (n_group0 > 1) t.push("om:i:"+ori_mapQ);
if (hits[reported_i].lifted_str) t.push("lt:Z:" + hits[reported_i].lifted_str);
buf2.push(t);
// stage the hits generated from the XA tag
var cnt = 0;
var rg = (m = /\t(RG:Z:\S+)/.exec(line)) != null? m[1] : null;
for (var i = 0; i < hits.length; ++i) {
if (opt.verbose >= 5) print(obj2str(hits[i]));
if (hits[i].g != reported_g || i == reported_i) continue;
if (!opt.show_pri && idx_alt[hits[i].ctg] == null) continue;
var s = [t[0], 0, hits[i].ctg, hits[i].start+1, mapQ, hits[i].cigar, t[6], t[7], t[8]];
// print sequence/quality and set the rev flag
if (hits[i].rev == hits[reported_i].rev) {
s.push(t[9], t[10]);
s[1] = flag | 0x800;
} else {
s.push(rs, rq);
s[1] = (flag ^ 0x10) | 0x800;
}
s.push("NM:i:" + hits[i].NM);
if (hits[i].lifted_str) s.push("lt:Z:" + hits[i].lifted_str);
if (rg != null) s.push(rg);
buf2.push(s);
}
}
print_buffer(buf2, fp_hla, hla);
file.close();
if (fp_evi != null) fp_evi.close();
if (fp_hla != null)
for (var h in fp_hla)
fp_hla[h].close();
buf.destroy();
aux.destroy();
// print weight of each contig
if (opt.pre != null) {
var fpout = new File(opt.pre + '.ctw', "w");
var weight_arr = [];
var weight_hla = [];
for (var ctg in weight_alt) {
var w = weight_alt[ctg];
weight_arr.push([ctg, w[6], w[7], w[8],
w[0], w[1].toFixed(3), w[2].toFixed(3), w[1] > 0? (w[2]/w[1]).toFixed(3) : '0.000',
w[3], w[4].toFixed(3), w[5].toFixed(3), w[4] > 0? (w[5]/w[4]).toFixed(3) : '0.000']);
weight_hla.push([ctg, w[4] > 0? w[5]/w[4] : 0]);
}
weight_arr.sort(function(a,b) {
return a[1] < b[1]? -1 : a[1] > b[1]? 1 : a[2] != b[2]? a[2] - b[2] : a[0] < b[0]? -1 : a[0] > b[0]? 1 : 0;
});
for (var i = 0; i < weight_arr.length; ++i) {
if (weight_arr[i][1] == '~') weight_arr[i][1] = '*';
fpout.write(weight_arr[i].join("\t") + '\n');
}
fpout.close();
}
}
bwa_postalt(arguments);