Data from HTG

[jbague]

Hi,

I have a question for dviraran and the community that I'm hoping you can help with. I have expression data from an experiment using the HTG methodology. The data is collected in two steps: the first step is similar to a microarray, where the probe is separated from other components in a filtration step, and the second step involves PCR amplification. More information about the method is available on (https://www.htgmolecular.com/).

My question concerns normalization. The HTG platform only provides raw data and CPM normalization. I don’t have enough information about each gene to apply other normalization methods, like those used in microarrays. Could you provide guidance on how to proceed with XCell functions?

Thank you in advance for any advice or suggestions. I appreciate your help!

Best regards!