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Problem with PathSeqPipelineSpark : Not generating bam #8909

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martitroiani opened this issue Jul 11, 2024 · 0 comments
Open

Problem with PathSeqPipelineSpark : Not generating bam #8909

martitroiani opened this issue Jul 11, 2024 · 0 comments

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@martitroiani
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Hello,
I'm using gatk 4.2.2.0 with PathSeqPipelineSpark but I'm having some problems.
For some samples I have the generation of the outuput.bam file while for others no.

gatk --java-options "-Xmx750G" PathSeqPipelineSpark
--input output.bam
--filter-bwa-image ${imagehuman}
--kmer-file ${pathseqdb}/host.hss
--min-clipped-read-length 60
--microbe-dict ${bactdict}
--microbe-bwa-image ${pathseqdb}/bacteria_only.fa.img
--taxonomy-file $taxdb
--output ${outpath}pathseq.complete0.8.bam
--scores-output ${outpath}/pathseq.complete0.8.csv
--is-host-aligned false
--filter-duplicates false
--min-score-identity .8

The path to the related file are correct so I was wondering if someone can help me.

This is the error
A USER ERROR has occurred: Couldn't write file pathseq.complete0.8.bam because writing failed with exception No FileSystem for scheme "null"

Best,
Martina

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