A Jython script for Fiji/ImageJ to perform Difference of Gaussians on fluorescent stack and plot the result.
This script will open a single channel fluorescent stack, perform the Difference of Gaussians and then plot all found points with the orthoslice view
- cellDiameter : select the cell/nucleus diameter (in unit of the Image Stack)
- minPeak : min intensity for a peak to be considered
- plotType : plot Points, Icospheres or limit to the first 3495 points due to some slowdown with Icospheres
- cellIcosophere : size of the point/icosphere use to visualize each detected point.
- Apply 3D Watershed segmentation using the detected points has a seed (see 3D Watershed plugin )
- Allow multiple channels in the stack
- Add a dialog for the parameters
- Add interface to measure nucleus/cell parameters