Amplify C1q trout gene for multiplex BS seq (PacBio)

[sr320]

Here are details regarding barcodes into PCR products once general primer pair is determined to be viable

https://d.pr/f/K2we5f

[kubu4]

So, what's the process here?

Initially, I thought we were just going to generate PCR products and send them off.

Instead, is the workflow like this?

1. Check current BS primers on pooled BS DNA.
2. If Step 1 works, then design PacBio barcoded primers.
3. Run BS PCRs on individual BS DNA samples with new PacBio barcoded primers.
4. Purficy PCR products.
5. Send to sequencing facility.

[sr320]

Yes, I believe so.

…

On Thu, Aug 29, 2024 at 9:44 AM kubu4 ***@***.***> wrote: So, what's the process here? Initially, I thought we were just going to generate PCR products and send them off. Instead, is the workflow like this? 1. Check current BS primers on pooled BS DNA. 2. If Step 1 works, then design PacBio barcoded primers. 3. Run BS PCRs on individual BS DNA samples with new PacBio barcoded primers. 4. Purficy PCR products. 5. Send to sequencing facility. — Reply to this email directly, view it on GitHub <https://urldefense.com/v3/__https://github.com/RobertsLab/resources/issues/1956*issuecomment-2318340039__;Iw!!K-Hz7m0Vt54!iR2R5kXSbknheO3U9PnhrLq5FuSIqS3MEgopJ4Sa8s3L9-Acuc9OruNIeEi6NUoIGYOmUxU9cmzIzAua6e3mW1E$>, or unsubscribe <https://urldefense.com/v3/__https://github.com/notifications/unsubscribe-auth/ABB4PN52NQXYVJV5AJIT32LZT5FWHAVCNFSM6AAAAABNKZCRAOVHI2DSMVQWIX3LMV43OSLTON2WKQ3PNVWWK3TUHMZDGMJYGM2DAMBTHE__;!!K-Hz7m0Vt54!iR2R5kXSbknheO3U9PnhrLq5FuSIqS3MEgopJ4Sa8s3L9-Acuc9OruNIeEi6NUoIGYOmUxU9cmzIzAua5KSJR3o$> . You are receiving this because you authored the thread.Message ID: ***@***.***>