Invalid Spectrum File Path

[Rachitha17]

log_2024-10-03_13-13-33.txt

I followed all steps according to the tutorial, but i cannot see the spectrum after i open the PDV viewer, I can see all the other results but not the spectrum, kindly help

[fcyu]

For the error in the first screenshot and the log file, the "assign glycans with FDR" was enabled by mistake. Could you disable that one and rerun?

For the FragPipe-PDV errors, could you try again after rerunning FragPipe?

Thanks,

Fengchao

[Rachitha17]

Thank you so much for the immediate response, I am very thankful!

I did as you mentioned and the error on the first screenshot got solved, as I did not get that error again but I still get Invalid spectrum file path

[fcyu]

Kai @KaiLiCn can you take a look?

Thanks,

Fengchao

[KaiLiCn]

Hi, looks like you failed to finish your analysis in the beginning. Did you finish the analysis after changing the parameter? Please make sure your last output is something like this:

If you still get the same error message, please share the new log file with me.

Thanks,
Kai