diff --git a/zarrs/src/array/storage_transformer/chunk_manifest_json/chunk_manifest_storage_transformer.rs b/zarrs/src/array/storage_transformer/chunk_manifest_json/chunk_manifest_storage_transformer.rs
index f05d4ed3..d98a097f 100644
--- a/zarrs/src/array/storage_transformer/chunk_manifest_json/chunk_manifest_storage_transformer.rs
+++ b/zarrs/src/array/storage_transformer/chunk_manifest_json/chunk_manifest_storage_transformer.rs
@@ -197,7 +197,10 @@ struct ChunkManifestJsonImplStorageTransformer<TStorage: ?Sized> {
 
 impl<TStorage: ?Sized> ChunkManifestJsonImplStorageTransformer<TStorage> {
     fn key_to_manifest_value(&self, key: &StoreKey) -> Option<&ChunkManifestValue> {
-        let root = strip_root_prefix(self.path.as_str());
+        let mut root = strip_root_prefix(self.path.as_str());
+        if cfg!(windows) {
+            root = root.replace('\\', "/");
+        }
         let relative_key = key
             .as_str()
             .strip_prefix(&root)